HiFi sequencing with PacBio service providers delivers affordable microbiome and metagenomic data that is second to none
Connect with a PacBio scientist More samples, one SMRT Cell HiFi sequencing is the ideal long-read technology for multiplex sequencing. The high base accuracy allows confident identification of indexes, plus the on-instrument demultiplexing option simplifies and streamlines downstream data analysis. All...
these platforms are concentrated in centralised sequencing facilities—few of which are in sub-Saharan Africa. Although this situation is improving26, deficits in local sequencing capacity still impel many small- and medium-sized labs to ship samples internationally for sequencing. This reduces country ...
Low-input Low-cost amplification-free library-production method for PacBio Long-read sequencing All the codes for LILAP are available in the 'code.sh' file. The installation of the software should be conducted with the correct versions specified in the accompanying paper. Links for the single-...
When the sequencing depth is relatively low, such as ~20×, KSNP can still maintain good performance (Supplementary Tables 7 and 8). Table 1 Performance of KSNP and the four state-of-the-art haplotyping tools on PacBio CLR and HiFi datasets Full size table Table 2 Performance of KSNP and...
The technique, known as HGAP (Hierarchical Genome Assembly Process), uses PacBio's single molecule, real-time DNA sequencing platform, which generates reads that can be up to tens of thousands of nucleotides long, even longer than those provided by the workhorse technology of theHuman Genome Pro...
Furthermore, while the Illumina short-read sequencing technology has accounted for >95% of the published RNA-seq data available on the Short Read Archive (SRA), the long-read cDNA sequencing and direct RNA-seq technologies ushered by Pacific Biosciences (PacBio) and Oxford Nanopore (ONT) may ...
High-accuracy long-read amplicon sequences using unique molecular identifiers with Nanopore or PacBio sequencing Article 11 January 2021 Evaluation of a high-throughput, cost-effective Illumina library preparation kit Article Open access 05 August 2021 Handling of targeted amplicon sequencing data ...
Various methods are available for single nucleotide polymorphism (SNP) genotyping, each with their benefits and limitations. While SNP genotyping on a genome-scale is currently dominated by microarray analysis or massively parallel sequencing, assessment of a targeted selection of SNPs (or single nucleot...