Select a country first to see the price Proteintech Guarantee Overview Tested Applications Product Information Publications (1014) Protocols Reviews (9) Print datasheet MSDS Tested Applications Positive WB detected in A431 cells, SMMC-7721 cells, MCF-7 cells, rat colon tissue, HEK-293 cells, ...
Proteintech North America (HQ) Proteintech Group, Inc 5500 Pearl Street, Suite 400 Rosemont, IL 60018, USA 1-888-478-4522 proteintech@ptglab.com Proteintech Europe Transmission (6th Fl) 6 Atherton Street M3 3GS, Manchester, UK (+44) 161 839 3007 europe@ptglab.com Wuhan Sanying D3-3,...
Proteintech Group, Inc 5500 Pearl Street, Suite 400 Rosemont, IL 60018, USA 1-888-478-4522 proteintech@ptglab.com Proteintech Europe Transmission (6th Fl) 6 Atherton Street M3 3GS, Manchester, UK (+44) 161 839 3007 europe@ptglab.com Wuhan Sanying D3-3, No.666 Gaoxin Avenue Wuhan Ea...
p53-C-term-Trap Agarose is an affinity resin for IP of p53. It consists of a p53 Nanobody/ VHH coupled to agarose beads and that binds to the C-terminus of p53.
To investigate the ISCU/p53 protein status in clinical hepatocellular carcinoma samples, rabbit anti-ISCU antibody (14812-1-AP, Proteintech, Chicago, IL, USA), mouse anti-p53 antibody (OP140, Merck Millipore, Darmstadt, Germany), or rabbit anti-ferritin heavy chain (FTH1) antibody (sc-25617,...
总蛋白经聚丙烯酰胺凝胶电泳(上样量每孔20 μg)分离后转膜,用50 g/L脱脂奶粉封闭2 h后,洗膜3次,用抗P53(美国Proteintech公司产品)、抗PARP(美国Cell Signaling公司产品)及抗caspase-3(美国Cell Signaling公司产品)的一抗4 ℃孵育过夜,洗膜3次,用辣根过氧化物酶偶联的二抗(美国Proteintech公司产品)室温孵育1 h...
上海碧云天生物技术有限公司,p53 siRNA 和 progerin siRNA 均购自广州锐博生物技术有限公司,p53 抗体(鼠源)购自英国 Abcam 公司,progerin 抗体购自美国 Santa 公司,p53 抗体 ( 兔源 )、辣根过氧化物酶(HRP)标记的山羊抗鼠、山...
使用针对mdm4(17914-1-ap;proteintechgroup公司)、mdm2(smp14,圣克鲁斯生物技术公司)、ck1α(17125-1-ap;proteintechgroup公司)、磷酸-ser166mdm2(]3521,细胞信号传导技术公司(cellsignalingtechnology))、磷酸-ser15p53(]9284;细胞信号传导技术公司)、乙酰基-lys382p53(]2525;细胞信号传导技术公司)、p21(]2946;细胞...
同时为了检测p53蛋白和β-肌动蛋白,将封闭膜与兔p53抗体(LifeScience,USA)1:400稀释液及鼠β-肌动蛋白抗体(ProteinTech,USA)1:2000稀释液孵育。室温孵育2小时后,用TBST缓冲液洗涤5次。然后,在室温下分别用鼠His-tag二抗标记辣根过氧化物酶的鼠IgG抗体(ProteinTech,1:10000稀释液),以及兔p53二抗辣根过氧化物酶...
The immunoprecipitations were probed with antibodies against p53 (Proteintech) or normal rabbit IgG (Invitrogen) overnight at 4 °C and protein A+G Agarose/Salmon Sperm DNA. Subsequently, we washed the DNA-protein complex three times at 4 °C. The ChIP products for PCR detection after DNA ...