ASCE(Additional Strand Catalytic E)蛋白是 P-loop NTPase 中最主要、功能最多样的一类NTPase。生物信息学分析发现古菌中存在一类保守的,而且是古菌特有的P-loop NTPase。在冰岛硫化叶菌Sulfolobus islandicus Rey15A中,其编码基因为SiRe_1432,与Hjc编码基因相邻,且为必须基因。该ATPase的N-端有一个保守的PIN(Pil ...
(2013) The unconventional P-loop NTPase OsYchF1 and its regulator OsGAP1 play opposite roles in salinity stress tolerance. Plant, Cell & Environment , n/a-n/a Online publication date: 1-May-2013. /Cheung MY, Li MW, Yung YL, Wen CQ, Lam HM (2013) The unconventional P‐loop NTPase ...
A unique feature of all eukaryotic and certain bacterial KAP NTPases is the presence of two or four transmembrane helices inserted into the P-loop NTPase domain. These transmembrane helices anchor KAP NTPases in the membrane such that the P-loop domain is located on the intracellular side. We...
Careful and quantitative analysis of the kinetics and thermodynamics of these NTPases with nucleotides and exchange factors, provides an understanding of how their interactions control the NTPase system in a manner that is specifically adapted to achieve the biological role(s) of these proteins. 展开...
(29) regarding the nucleotide specificity for wild type RecA NTPase activity. Hydrolysis of NTPs by either protein required single-stranded DNA as a cofactor. Table III:. NTPase nucleotide specificity for both wild type RecA and the Pro67→ Trp mutant proteins NTPase assays were performed as des...
Reconstruction of Primordial P-loop NTPase PrecursorsIn collaboration with: Agnes Toth-Petroczy, Alexander Goncearenco and Igor Berezovsky (inference of the ancestral P-loop sequence) and Lin Yu-Ru and David Baker (computational protein design).Short functional peptides are likely to have served as...
WalkerAmotifThe P-loop NTPases are involved in diverse cellular functions. Members of the P-loop NTPase superfamily are characterized by presence of a highly conserved sequence pattern GxxxxGKS/T, known as Walker A motif. This motif adopts an archetypal P-loop conformation which allows ...
Structural Characterizations of a Novel P-loop NTPase OsYchF1 and Its Regulator OsGAP1Our laboratory previously identified a rice () C2 domain containing protein OsGAP1, using reverse genetic suppression subtraction library method. OsGAP1 plays a positive role in plant disease resistance. After that,...
Careful and quantitative analysis of the kinetics and thermodynamics of these NTPases with nucleotides and exchange factors, provides an understanding of how their interactions control the NTPase system in a manner that is specifically adapted to achieve the biological role(s) of these proteins.Chin,...
P-loop kinaseSequences and structures of all P-loop-fold proteins were compared with the aim of reconstructing the principal events in the evolution of P-loop-containing kinases. It is shown that kinases and some related proteins comprise a monophyletic assemblage within the P-loop NTPase fold....