PNAS.2018-Function and crystal structure of the dimeric P-loop ATPase CFD1 coordinating an exposed [4Fe-4S] cluster for transfer to apoproteins.pdf 2018-10-25上传 暂无简介 文档格式: .pdf 文档大小: 1.26M 文档页数: 10页 顶/踩数:
OsGAP1 enhances both the ATPase and GTPase activities of OsYchF1. In collaboration with Prof. Kam-Bo Wong's laboratory, my project is to solve both of OsYchF1 and OsGAP1 3-dimension structures.Miao, Rui
Ruepp A, Wanner G & Soppa J (1998) A 71-kDa protein from Halobacterium salinarum belongs to a ubiquitous P-loop ATPase superfamily with head-rod-tail structure. Arch Microbiol 169: 1-9.Ruepp A , Wanner G & Soppa J ( 1998 ) A 71-kDa protein from Halobacterium salinarum belongs to a...
该ATPase的N-端有一个保守的PIN(Pil T N-terminal)结构域,因此被命名为PINA(for PIN-domain ATPase)。前期的研究结果证明SisPINA是Ca2+依赖的ATPase,具有HJ结构结合活性和Ca2+依赖的解旋酶活性。酵母双杂交实验和pull-down实验证明SisPINA与Hjc有物理上的相互作用,但具体的相互作用机制和体内的生物学意义仍不清楚...
Nucleotide binding proteins, including ras, elongation factor Tu, adenylate kinase, and the mitochondrial F1-ATPase have a glycine-rich motif known as the P-loop or the Walker A sequence (Walker, J. E., Saraste, M., Runswick, M. J., and Gay, N. J. (1982) EMBO J. 1, 945-951)...
The properties of Penicillium chrysogenum adenosine 5′-phosphosulfate (APS) kinase mutated at Ser-107 were examined. Ser-107 is analogous to a serine of the E. coli enzyme that has been shown to serve as an intermediate acceptor in the transfer of a phosphoryl group from ATP to APS. ...
As a side result, we determined the geometry of an Mn2+-ADP:AlF3 complex in a P-loop ATPase (Figure 5) and thus offered a solution to the long-standing controversy as to whether NDP:AlF3 complexes could form in the catalytic site of P-loop NTPases under appropriate conditions or whether...
The AAA-ATPase VCP/p97/Cdc48 unfolds proteins by threading them through its central pore, but how substrates are recognized and inserted into the pore in diverse pathways has remained controversial. Here, we show that p97, with its adapter p37, binds an internal recognition site (IRS) within...
The phosphate-binding loop (P-loop) is a conserved sequence motif found in mononucleotide-binding proteins. Little is known about the structural dynamics of this region and its contribution to the observed nucleotide binding properties. Understanding the underlying design principles is of great interest...
MglA, belonging to TRAFAC class of P-loop NTPases, possesses a threonine at the position equivalent to Walker B aspartate in eukaryotic Ras-like GTPases. To resolve the identity of the Walker B residue in MglA, we carried out a comprehensive analysis of Mg coordination on P-loop NTPase ...