P-loopNTPasesWalkerAmotifThe P-loop NTPases are involved in diverse cellular functions. Members of the P-loop NTPase superfamily are characterized by presence of a highly conserved sequence pattern GxxxxGKS/T, known as Walker A motif. This motif adopts an archetypal P-loop conformation which ...
However, structures of such stable complexes may not reflect the authentic short-lived states during enzymatic hydrolysis, nor do they cover any members of the most prevalent nucleotide-binding fold, the P-loop NTPases34. We report here the 2.6-Å cryo-EM structure of the human ATPase p97 ...
p pBackground/p pThe P-loop NTPases constitute one of the largest groups of globular protein domains that play highly diverse functional roles in most of the organisms. Even with the availability of nearly 300 different Hidden Markov Models representing the P-loop NTPase superfamily, not many ...
Careful and quantitative analysis of the kinetics and thermodynamics of these NTPases with nucleotides and exchange factors, provides an understanding of how their interactions control the NTPase system in a manner that is specifically adapted to achieve the biological role(s) of these proteins.;The ...
Hydrolysis of nucleoside triphosphates (NTPs), such as ATP or GTP, by so-called P-loop fold nucleoside triphosphatases (also known as Walker NTPases) is one of the key enzymatic reactions. P-loop NTPase domains drive the activity of rotary ATP synthases, DNA and RNA helicases, kinesins an...
The DNA helicase (gp31) of MED16 belongs to the SSL2 superfamily (cl34083) and DNA primase/replicative helicase (gp68) contains AAA_25 domain (pfam13481) of the P-loop_NTPase superfamily (cl38936). The MED16 genes associated with DNA RRR, with the exceptions of ORF03 and ORF68, are...