ASCE(Additional Strand Catalytic E)蛋白是 P-loop NTPase 中最主要、功能最多样的一类NTPase。生物信息学分析发现古菌中存在一类保守的,而且是古菌特有的P-loop NTPase。在冰岛硫化叶菌Sulfolobus islandicus Rey15A中,其编码基因为SiRe_1432,与Hjc编码基因相邻,且为必须基因。该ATPase的N-端有一个保守的PIN(Pil ...
One such motif, probably the most obvious one, is the P-loop (Walker A motif) that typically binds the transferred phosphate moiety of ATP. The P-loop's extended motif comprises a β-strand and an α-helix connected by the P-loop – a glycine-rich phosphate-binding loop. The P-loop ...
However, structures of such stable complexes may not reflect the authentic short-lived states during enzymatic hydrolysis, nor do they cover any members of the most prevalent nucleotide-binding fold, the P-loop NTPases34. We report here the 2.6-Å cryo-EM structure of the human ATPase p97 ...
Structural Characterizations of a Novel P-loop NTPase OsYchF1 and Its Regulator OsGAP1 Our laboratory previously identified a rice () C2 domain containing protein OsGAP1, using reverse genetic suppression subtraction library method. OsGAP1 pl... R Miao 被引量: 0发表: 2015年 The relationship betw...
P-loopNTPasesWalkerAmotifThe P-loop NTPases are involved in diverse cellular functions. Members of the P-loop NTPase superfamily are characterized by presence of a highly conserved sequence pattern GxxxxGKS/T, known as Walker A motif. This motif adopts an archetypal P-loop conformation which ...
OsYchF1 belongs to YchF subfamily of Obg family in TRAFAC superclass of P-loop GTPases, and is involved in plant defense response pathway as a negative regulator. OsGAP1 enhances both the ATPase and GTPase activities of OsYchF1. In collaboration with Prof. Kam-Bo Wong's laboratory, my ...
Careful and quantitative analysis of the kinetics and thermodynamics of these NTPases with nucleotides and exchange factors, provides an understanding of how their interactions control the NTPase system in a manner that is specifically adapted to achieve the biological role(s) of these proteins.;The ...
Aravind L, Iyer LM, Leipe DD, Koonin EV: A novel family of P-loop NTPases with an unusual phyletic distribution and transmembrane segments inserted within the NTPase domain. Genome Biol 2004, 5 (5) : R30.Aravind L, Iyer LM, Leipe DD, Koonin EV: A novel family of P-loop NTPases ...
Careful and quantitative analysis of the kinetics and thermodynamics of these NTPases with nucleotides and exchange factors, provides an understanding of how their interactions control the NTPase system in a manner that is specifically adapted to achieve the biological role(s) of these proteins.Chin,...
Pang, RuiQiu, JieqiQiu, JieqiQiu, JieqiQiu, JieqiLi, TengchaoLi, TengchaoBIOCHIMICA ET BIOPHYSICA ACTAThe regulation of lipid metabolism by ahypothetical P-loop NTPase and its impact on fecundity of the brown planthopper. Pang R,Qiu JQ,Li TC.et al. Biochimica et Biophysica Acta-General ...