OT-1 Mice 一、品系名称品系名称:OT-1 Mice品系背景:C57BL/6J毛色:黑色相关基因型:a/a 二、品系描述 T细胞是一种在免疫反应中起着关键作用的白细胞,主要有CD4+T细胞(辅助性T细胞)和CD8+T细胞(杀伤性T细胞)两种亚型。CD4+T细胞主要通过识别抗原并分泌细胞因子激活其他免疫细胞,从而协调免疫反应。与此相反,CD8+...
Table S1. Activated OT-1 cells kill growing and nongrowing SIINFEKL-pulsed B16 cells with approximately equal efficiencyova-B16 tumor volume (control mice)bOT
with cells transgenic for ovalbumin or by direct administration of ovalbumin. These mice are often used to study CD8+ T cell response to antigen, positive selection, and in any research requiring CD8+ T cells of defined specificity. Like most TCR transgenics, these mice are somewhat immuno...
Interestingly, loss of imprinting of Cdkn1c occurs in ΔKcnq1ot1 mice (Mancini-Dinardo et al., 2006). However, the significance of H3K27me3 enrichment both at 5′ and 3′ ends of the Cdkn1c gene in ΔKcnq1ot1 mice remains to be investigated. The Kcnq1ot1 promoter, which is ...
The resulting phenotypes in mouse models are remarkably different: Kmt2c depleted mice die after birth without morphological alterations, whereas Kmt2d KO mice die during embryonic development, at embryonic stage E9.5, due to defects in heart development, adipogenesis, and myogenesis. In addition, ...
16 Acampora D, Mazan S, Avantaggiato V et al: Epilepsy and brain abnormalities in mice lacking the Otx1 gene. Nat Genet 1996; 14: 218–222. 17 Robinson PD, Schutz CK, Macciardi F, White BN, Holden JJ: Genetically determined low maternal serum dopamine beta-hydroxylase levels and the ...
TTC staining was used to evaluate infarct size in mice. TUNEL was employed to assess cell apoptosis. QRT-PCR was performed to detect the expression of KCNQ1OT1 and miR-26a-5p. The formation of autophagosomes in cells was detected by immunofluorescence. Caspase3 activity was detected by the ...
Furthermore, to determine whether KCNQ1OT1 promoted growth of the OS cells in vivo, we utilized xenograft experiments by subcutaneously injecting stable KCNQ1OT1-knockdown or control U-2OS cells into nude mice. We found that the stable KCNQ1OT1-knockdown group exhibited significantly decreased th...
In an attempt to stimulate IKDC-like activity in bone-marrow-derived DCs (BMDCs), we stimulated the BMDCs with IL-2. These "DCs-IL-2" cells possessed an impressive anti-tumor effect in mice when injected s.c, mixed with M05 tumor cells.doi:10.1096/fasebj.22.1_supplement.1076.1QIN YANG...
品系名称:OT-1 Mice 品系背景:C57BL/6J 毛色:黑色 相关基因型:a/a 二、品系描述 OT1/OT2小鼠T细胞受体(TCR)转基因鼠,都是B6背景。 OT-1小鼠有Tcra-V2与Tcrb-V5基因的插入突变,CD8+ T细胞受体特异性识别OVA 257-264肽段,可用于研究多肽在CD8+T细胞对抗原的阳性选择和应答中的作用。和大多数转基因TCR一...