Then, these gRNAs and Cas9 mRNA were injected into mouse zygotes and implanted into pseudopregnant mice. A Sox2ot promoter-deleted mouse line was identified with complete deletion of promoter as well as deletion of exon 1 and exon 2. Sox2ot transcript was truncated with a lack of exon 1 ...
Transposon (de)repression and heterochromatin reorganization are dynamically regulated during cell fate determination and are hallmarks of cellular senescence. However, whether they are sequence specifically regulated remains unknown. Here we uncover that the KCNQ1OT1 lncRNA, by sequence-specific Hoogsteen ...
7a). CAL27 or control cells with KCNQ1OT1 knocked down were then injected subcutaneously into BALB/c male nude mice. One week after tumor cell inoculation, the nude mice bearing xenografts of CAL27 cells with control shRNA or those with shRNA against KCQ1OT1 (CAL27-sh-KCNQ1OT1) were...
2021年12月,在Nature上发表的题为“Low immunogenicity of LNP allows repeated administrations of CRISPR-Cas9 mRNA intoskeletal muscle in mice”的一项研究中,作者报告了一种新型脂质纳米颗粒,它能够通过重复的肌肉注射将Cas9 mRNA和sgRNA递送到骨骼肌中。...
上海交通大学系统生物医学研究院的蔡宇伽团队发表的“Lentiviral delivery of co-packaged Cas9 mRNA and a Vegfa-targeting guide RNA prevents wet age-related macular degeneration in mice”的论文报道过利用VLP递送CRISPR/Cas9 mRNA,实现安全和高效...
本研究采用了猪全基因组CRISPR/Cas9高通量筛选的方法挖掘乙型脑炎病毒(JEV)抗性关键宿主因子,制备猪全基因组敲除筛选工具,建立猪全基因组CRISPR/Cas9敲除筛选平台,为筛选猪重要性状的功能基因提供理论基础。本研究采用JEV筛选模型为例,为筛...
基因组学及功能基因Genomics & Functional Gene研究报告Research Reports水稻纹枯病菌谷氨酸合成酶RsGLT1基因的克隆及生物信息学分析Cloning and Bioinformatics Analysis of Glutamate SynthaseRsGLT1Geneof Rice Sheath Blight曾泉(Zeng Q.),史国英(Shi G.Y...
2024年4月3日,南京大学魏继武团队在JOURNAL OF EXPERIMENTAL & CLINICAL CANCER RESEARCH在线发表题为“Oncolytic adenovirus encoding apolipoprotein A1 suppresses metastasis of triple-negative breast cancer in mice”的研究论文,该研究构建了一...
英国MeiraGTx公司研究人员利用CRISPR/CAS9 技术生成 RetGC 敲除 (RetGC KO) 视网膜类器官,iPSC衍生视网膜类器官分化后,将RetGC KO 视网膜类器官与同一细胞系的野生型类器官进行对比研究。总共设计了四种 AAV 载体来测试RetGC 蛋白在光感受器中的恢复情况,所有载体...
在兔子模型中发现病毒复制,但是没有临床表现,所以少量药物研究在使用。人DDP4转基因小鼠开始被使用,类似于SARS-CoV,在小鼠肺传代产生适应性,进而产生临床表现。CRISPR/Cas9也被用于产生特定基因突变DDP4的转基因鼠。 灵长类动物 小动物模型作为冠状病毒感染的模型得到了更彻底...