Then, a LPS-induced ARDS mouse model was con-structed, with a series of gain- or loss-of-function experimentsconducted to evaluate the lung function and neutrophil extra-cellular trap (NET) formation in lung tissue and determine theneutrophil number, myeloperoxidase (MPO) activity, and in-f ...
Because the OT-1 mouse was made by micronuclear injection of genomic DNA, which did not contain unique transgene non-coding sequence, we based a quantitative PCR strategy on the DNA sequence encoding the rearranged VDJ sequences corresponding to the CDR3 region of the TCR Beta chain. As genom...
5d–g). Similarly, knockdown of KCNQ1OT1 also promoted the expression of miR-34c-5p and inhibited the expression of ALDOA in tumor tissues from a subcutaneous xenograft mouse model (Supplementary Fig. 5h). These results validated that KCNQ1OT1 promotes OS growth and the Warburg effect via ...
However, according to the last discoveries made by Dorighi and Rickles, since these studies employed a mouse model which does not mimic the haploinsufficiency, it is possible that it might not completely recapitulate KS pathogenesis, as the complete removal of the entire gene product leads to a ...
(Supplementary Fig.1). To conduct this screen, we used an existing transgenic mouse model, where exons 1 and 2 of theCdkn1cimprinted gene were replaced with the PGK-neomycin resistance cassette (Cdkn1c+/ΔneoR)18(Supplementary Fig.2a). Our strategy was predicated upon silencing of the ...
RESULTS :KCNQ1OT1 was up-regulated while miR-26a-5p was decreased in MI patients, I/R mouse and H/R-induced cell model. KCNQ1OT1 knockdown inhibited cell autophagy and protected cardiomyocytes from apoptosis by up-regulating miR-26a-5p. Either KCNQ1OT1 knockdown or miR-26a-5p mimics ...
A myocardial IR injury mouse model was established in vivo and an in vitro cardiomyocyte model was induced by hypoxia/Reoxygenation exposure. Next, gain- and loss-of-function experiments were employed in order to measure the viability and apoptosis of cardiomyocytes and the area of ischemic infarct...
human,mouse WB,IF Adv Sci (Weinh) OTUD5 Protects Dopaminergic Neurons by Promoting the Degradation of α-Synuclein in Parkinson's Disease Model Authors - Xiaomeng Song View Article human,mouse WB Int J Radiat Oncol Biol Phys USP11 exacerbates radiation induced pneumonitis by activating endothelial ...
ofKcnq1ot1transcription, preserved in knockdown assays, and not the RNA product per se, that plays a role in the imprinting process [60]. Consistent with our finding, it was reported that the depletion of Kcnq1ot1, through short hairpin RNAs, did not cause loss of imprinting in mouse ...
8 However, pmel cells are self-tolerant mouse gp100 (mgp100)-specific CD8+ T cells, which do not protect against mgp100-expressing B16 tumors unless stimulated via infection with a vaccinia virus expressing the human gp100.34 The affinity of the pmel cells to their cognate antigen is ...