Like most TCR transgenics, these mice are somewhat immunodeficient. 五、验证数据 1、OT-I小鼠生长情况正常 图1. 雌性和雄性 OT-I 小鼠的体重变化情况。 雌性和雄性 OT-I 小鼠的体重变化均与野生型相似,生长情况正常。 2、OT-I 小鼠 CD4+ T 细胞发育异常 图2. OT-I 小鼠T细胞亚群检
Elimination and re-induction of OVA expressing hepatocytes in OVA_X_OT-1 mice.Marcin CebulaAaron OchelUpneet HillebrandMarina C. PilsReinhold SchirmbeckHansjörg HauserDagmar Wirth
with cells transgenic for ovalbumin or by direct administration of ovalbumin. These mice are often used to study CD8+ T cell response to antigen, positive selection, and in any research requiring CD8+ T cells of defined specificity. Like most TCR transgenics, these mice are somewhat immuno...
However, more recently, learning and memory tasks assessed in the same Ehmt1+/− mice in a low-stress and unaversive environment showed unhindered general learning deficits. The authors suggested that the previous result could be due to enhanced anxiety in Ehmt1+/− mice and only to a ...
Furthermore, to determine whether KCNQ1OT1 promoted growth of the OS cells in vivo, we utilized xenograft experiments by subcutaneously injecting stable KCNQ1OT1-knockdown or control U-2OS cells into nude mice. We found that the stable KCNQ1OT1-knockdown group exhibited significantly decreased th...
Results The expression of lnc-Kcnq1ot1 was significantly upregulated during osteoblast differentiation but downregulated in the bone tissues of osteoporotic mice and during osteoclast differentiation (P<0.05). Silencing lnc-Kcnq1ot1 obviously decreased the expression of Bglap and Alp (P<0.05) and ...
Using C57BL/6 J mice and primary mouse hepatocytes were conducted to establish HIRI model in vivo and in vitro. Cell viability was examined using CCK-8 assay and EdU assay. Flow cytometric analysis was performed to evaluate the pyroptosis. Dual-luciferase reporter assay was employed to verify ...
which results in protein-coding gene silencing. In mice, paternal inheritance of aKcnq1ot1ICR deletion leads to paternal reactivation of imprinted genes within the domain at midgestation12. Similarly, paternal transmission ofKcnq1ot1ncRNA truncations result in paternal allelic reactivation in midgestat...
Figure 1. KCNQ1OT1 Is Downregulated in the VSMCs of Mice with Intimal Hyperplasia and in the Process of VSMC Proliferation The vein graft model (VG) was constructed in mice (n = 25) to introduce intimal hyperplasia. (A and B) After 0, 1, 2, 3, and 4 weeks (n = 5 at each ...
品系名称:OT-1 Mice 品系背景:C57BL/6J 毛色:黑色 相关基因型:a/a 二、品系描述 OT1/OT2小鼠T细胞受体(TCR)转基因鼠,都是B6背景。 OT-1小鼠有Tcra-V2与Tcrb-V5基因的插入突变,CD8+ T细胞受体特异性识别OVA257-264肽段,可用于研究多肽在CD8+T细胞对抗原的阳性选择和应答中的作用。和大多数转基因TCR一...