OT-1小鼠是体内CD8+T特异性识别OVA OT-11小鼠是体内CD4+T特异性识别OVA 表达OVA的肿瘤打给小鼠,再给小鼠OT-1的CD8+T,相当于给小鼠肿瘤特异性T细胞。 OT-II: (CD4+---ovalbumin 323-339 ) These transgenic mice express the mouse alpha-chain and beta-chain T cell receptor that pairs with the CD4 ...
Background Transgenic TCR mice are often used experimentally as a source of T cells of a defined specificity. One of the most widely used transgenic TCR models is the OT-1 transgenic mouse in which the CD8+ T cells express a TCR specific for the SIINFEKL peptide of ovalbumin presented on...
A PCR strategy was developed to identify OT-1 positive transgenic mice, which express a T cell receptor (TCR) specific for the OVA peptide SIINFEKL, from wild-type littermates. Primers were designed in the VDJ region of the OT-1 TCRβ chain (Figure1) to yield a 238 bp product. Figu...
Thesetransgenic miceexpress the mouse alpha-chain and beta-chain T cell receptor that pairs with t...
OT-II: (CD4+---ovalbumin 323-339 )These transgenic mice express the mouse alpha-chain and beta...
OT-1 transgenic mice expressing a H-2Kb/OVA-specific TCR on the C57BL/6J background were bred in house until 6–15 weeks of age and used to obtain OT-1 CD8+ T cells. C57BL/6J mice were purchased from Envigo. C57BL/6J expressing the CD45.1 or CD45.1/2 congenic markers were bred...
with cells transgenic for ovalbumin or by direct administration of ovalbumin. These mice are often used to study CD8+ T cell response to antigen, positive selection, and in any research requiring CD8+ T cells of defined specificity. Like most TCR transgenics, these mice are somewhat immuno...
From:Peripheral antigen display by lymph node stroma promotes T cell tolerance to intestinal self Nature Immunology (Nat Immunol)ISSN1529-2916(online)ISSN1529-2908(print)
Induction of oral tolerance (OT) in T cell receptor transgenic mice: W.G. Thaggard, M. Tomasi, P.A. Dean, C.T. Weaver, C.O. Elson. Departments of Medicine, Surgery and Pathology, University of Alabama at Birmingham, ALInduction of oral tolerance (OT) in T cell receptor transgenic mi...
近日,南澳大利亚大学周新福教授、昆明医科大学第二附属医院熊静等人在Metabolic Brain Disease期刊发表了题为:Long term high fat diet induces metabolic disorders and aggravates behavioral disorders and cognitive deficits in MAPT P301L transgenic mice的...