The researchers were surprised to discover they could use the on-off switch to target the majority of genes in the human genome, even those that don’t make proteins. The UCSF/MIT team wanted to get a sense of whether CRISPRoff would be applicable to m...
“We identified two key points: First, on the fungal side, thatCt3activates the ABA-BOT biosynthesis gene cluster; and second, on the plant side, thatCt3induces the host plant’s ABA signaling pathways, through which the fungus inhibits plant growth,” explained Hiruma. The re...
Bacterial transcription: Turning the switch on gene expressionThe article discusses the structural changes of the metabolite-binding riboswitches to regulate transcription or translation of downstream genes to switch on gene expression.doi:10.1038/nrmicro3356Du Toit...
Here, using the converse behavioural states of fear extinction and its context-dependent renewal as a model in mice, we show that bi-directional transitions between states of high and low fear are triggered by a rapid switch in the balance of activity between two distinct populations of basal ...
Researchers at the University of California, San Diego and Oak Ridge National Laboratory have discovered how a genetic circuit in HIV controls whether the virus turns on or stays dormant, and have succeeded in forcing the virus towards dormancy, a findin
In conclusion, we have identified an on/off switch, CGA, that controls dense-core secretory granule biogenesis in endocrine cells. The synthesis of CGA itself can be regulated by hormones. Thus, CGA serves an important higher-order physiological role in controlling hormone secretion through regulatin...
Secretory granule biogenesis and chromogranin A: master gene, on/off switch or assembly factor? Secretory granules are found in specialized cell types, including endocrine cells, suggesting that a coordinated programme of gene expression is involved i... R Day,SU Gorr - 《Trends in Endocrinology ...
In contrast, the Ad vector containing the tet-on system increased gene expression by only 2- to 28-fold and required about two-log orders higher concentration of an inducer (doxycycline) to switch on the gene expression, compared with the Ad vector containing the tet-off system. Ad vectors ...
Fleming’s research reaches beyond nature versus nurture and into the realm of epigenetics, the ability of environmental factors to switch genes on or off without altering the underlying structure of DNA. “This concept is not new,” Fleming says, “but it has gained a lot of interest in my...
A reversible RNA on-switch that controls gene expression of AAV-delivered therapeutics in vivoGuocai Zhong, Haimin Wang, Wenhui He, Yujun Li, Huihui Mou, Zachary J. Tickner, Mai H. Tran, Tianling Ou, Yiming Yin, Huitian Diao & Michael Farzan ...