Mahdieh, A. Shirkavand, H. Bagherian, M.A. Tabatabaiefar, A transversion mutation in non-coding exon 3 of the TMC1 gene in two ethnically related Iranian deaf families from different geographical regions; evide
(circRNAs). Chemically circular RNAs produced by nonsequential exon–exon back-splicing.Circular intronic long non-coding RNAs (ciRNAs). Circular, branched intronic RNAs resulting from stabilized introns after canonical splicing.Exon-intron circRNAs (EIciRNAs). A class of circular RNAs that retain un...
(H) Gene model: The Variant Explorer pipeline relies on 3 gene annotations for definition of exon and UTR boundaries: UCSC KnownGene, RefGene and Ensembl (Table S10). We use KnownGene as our default gene annotation (Tables2, S5a-b and S8a-b). 2d and Table S6) and we performed the M...
(1)“lariat-driven circularization”或者“exon skipping”; (2)“intron-pairing-driven circularization”或者“direct backsplicing”; (3)环状内含子RNA(ciRNAs)形成模式; (4)依赖于RBPs环化模式; (5)类似于可变剪切的可变环化模式; 5中不同的形成模型如下图所...
This probe is a 663-bp EcoRI fragment of the IGF-II cDNA =-=(40)-=- that recognizes an AvaII polymorphism near the 5�-part of exon 9 of the IGF-II gene (Fig. 1). In normal tissues, the 5�-part of exon 9 of the maternal allele of the IGF-II gene is specifically hypo...
Non-coding-RNA 李奇研发部经理基因芯片与生物信息领域从业9年 基因数量的矛盾 根据人类基因组序列预测，共3～4万个基因根据EST序列，获得10万个基因 大量垃圾序列存在的理由 CDS RegulaoryRNA hRNA RNA调控网络 RNA-RNARNA-DNARNA-蛋白质 蛋白间作用网络 代谢网络网络 DNA调控网络 DNA-蛋白质染色体修饰 其...
Non-coding RNA Non-codingRNA WhatisnoncodingRNA?Non-codingRNA(ncRNA)isaRNAmoleculethatfunctionswithoutbeingtranslatedintoaprotein HowmanyRNAsincells?mRNAProteinrRNAtRNASRP-RNAsnRNAsnoRNApRNAmicroRNA ??gRNARibozyme AntisenseRNATelomeraseRNA FunctionaldiversityofncRNAs Thefunctionofdiffenentsmallnon-codingRNAsby...
Tsuiji H, Yoshimoto R, Hasegawa Y, Furuno M, Yoshida M, Nakagawa S (2011) Competition between a noncoding exon and introns: gomafu contains tandem UACUAAC repeats and associates with splicing factor-1. Genes Cells: Devoted Mol Cell Mech 16(5):479–490 ...
removal of the promoter and transcriptional start site, abolishing exon–exon junctions, or removing the transcriptional termination site. Here, we describe the methodology to perform CRISPR/Cas9 KO of lncRNAs in vitro using electroporation as the method of transfection of presynthesized single guide RN...
Both the exon and promoter of the non-coding RNA RMRP were significantly mutated in multiple types of cancer (Fig. 1b, Extended Data Fig. 5c). Germline RMRP mutations cause cartilage–hair hypoplasia, and previous in vitro studies have shown that some somatic promoter mutations are functional16...