In particular, the following features were required for putative functional fusions: (1) presence of an intergenic junction (between two exons or between an exon and a cryptic exon); (2) a predicted in-frame co
Distinct requirement for NMB and GRP in itch transmission We generatedNmbKO mice using a gene targeting strategy (Fig.1A) and confirmed the absence ofNmbinNmbKO mice by PCR (Fig.1B) andin situhybridization (ISH) (Fig.1C). Although eGFP was fused in frame to the first coding exon ofNmb...
Open boxes indicate non-translated exon sequence and black boxes coding exon sequence. The two genes share a similar organization with seven exons. A major difference is the size of the first intron, which is >9 kb in the human gene but only ∼2 kb in its mouse ortholog. A BLAST ...
We determined the exon–intron structure of the CRX gene (Figure 3B) to facilitate detection of mutations in genomic DNA samples. Analysis of PAC and phage clones revealed three CRX coding exons separated by two introns of 1.2 kb and ∼3 kb, respectively. The introns interrupt the coding se...
Two BAC transgenes, with EGFP insertions into the third coding exon of Cryaa gene, were created: the intact αA-crystallin 148 kb BAC (αA-BAC) and αA-BAC(ΔDCR3), which lacks approximately 1.0 kb of genomic DNA including DCR3. Expression of EGFP in the majority of both BAC ...
The retained transcript of the intron region in most cases encodes amino acids in-frame with the neighboring exons [19]. Recent results have suggested that schistosomes create multiple protein variants by splicing micro-exon gene transcripts, which might be involved in immune evasion mechanisms [20]...
“The ORF Finder (Open Reading Frame Finder) is a graphical analysis tool which finds all open reading frames of a selectable minimum size in a user’s sequence or in a sequence already in the database” https://www.ncbi.nlm.nih.gov/orffinder/ PCR PRIMER DESIGN AND REACTION OPTIMISATION...
intergenic, Es: single-exon gene, Ei: initial exon, Et terminal exon, E0–E2: exons in phase 0–2, I0–I2: introns in phases 0–2 (subscript of T, TA, or TG denotes the last bp or two bp of the intron – this is used to prevent in-frame stop codons across splice junctions)...
can lead to in-frame exon skipping. PLoS ONE 12 (6): e0178700. https://doi/10.1371/journal. pone.0178700 Editor: Alfred S Lewin, University of Florida, UNITEDSTATES Received: March 25, 2017 Accepted: May 17, 2017 Published: June 1, 2017 ...
coding capability by one or more of the following methods were classified as transcripts of uncertain coding potential (TUCP): CPAT [23], threshold = 0.364; CPC [22], threshold = 0; Pfam [24]. For comparing to the Pfam database, the longest potential open reading frame (ORF) ...