Transient expression of cDNAs indicated that both types of alternatively spliced contain two in-frame AUGs functioning as alternate start sites for . Thus, transfections with exon X-lacking cDNAs resulted in the expression of Mr 33,000 and 31,000 proteins, detected by immunoprecipitation with anti...
The splicing machinery which positions a protein export complex near the exon-exon junction mediates nuclear export of mRNAs generated from intron-containi... H Gruffat,J Batisse,D Pich,... - 《Journal of Virology》 被引量: 184发表: 2002年 Overexpression of tumor necrosis factor (TNF)|[alp...
With respect to the structure of the hTPO gene, the present deletion suggests an alternate splicing of exon 16. The juxtaposition of exon 17 to exon 15 encoding the transmembrane domain leads to a shift in the reading frame. By the use of a different stop codon, the spliced mRNA generates...
The variable sequence is encoded by multiple versions of the ninth exon of M. sexta serpin gene-1. We present here the complete nucleotide sequence of M. sexta serpin gene-1, in which we have identified 12 alternate versions of exon 9. We have also isolated cDNAs corresponding to all of ...
Each cDNA contains a long open reading frame or the 5′ end of along open reading frame which ends several hundred nucleotides downstream on the viral genome. The 5′ untranslated regions are unusually long. Three mRNA species differing in their 5′ untranslated regions may encode for the ...
Among them, a 25 base pair deletion in the branch point of intron 32 of MYBPC3 is widespread, particularly affecting people of South Asian descent, with 4% of this population carrying the mutation. This polymorphic mutation results in skipping of exon 33 and a reading frame shift, which, ...
The predominant exon (based on the observed EST frequency) revealed a tissue distribution similar to that obtained using the reading frame as probe. Thus, it appears that one exon preferentially gives rise to mature mRNA in the human tissues examined. This exon is different from the one ...
Nonsense mutations within exon 1 of DMD do not result in severe DMD but instead lead to very mild BMD, due to an alternative initiation of translation at AUG codons within in exon 6. This leads to translation of a nearly full-length but N-terminal truncated dystrophin lacking the first ...