in development and in cancer.59LincRNAX-inactive specific transcript(Xist), which is responsible for X chromosome inactivation and gene dosage compensation, was discovered in the early 90s and is the first long
Clones 1 and 2 that harbor the insertion were expanded and analyzed for ThymoD exon3 and Bcl11b expression by real-time PCR. Expression abundance is shown by comparing Bcl11b to ARP transcript levels. Results show the mean ± SD (n = 4–7). As a first approach to examine potential ...
Therapeutic targeting of noncoding RNAs (ncRNAs), such as microRNAs (miRNAs) and long noncoding RNAs (lncRNAs), represents an attractive approach for the treatment of cancers, as well as many other diseases. Over the past decade, substantial effort has b
Herein, we identified a long noncoding transcript, namely, lymphocyte-specific protein 1 pseudogene 5 (LSP1P5), that modulates ECM component deposition in keloids. First, high-throughput transcriptome analysis showed that LSP1P5 was selectively upregulated in keloids and correlated with more severe ...
Location of lincRNAs and single-exon intergenic non-coding transcripts.Marc, P. HoeppnerAndrew, LundquistMono, PirunJennifer, R. S. MeadowsNeda, ZamaniJeremy, JohnsonGorel, SundstromApril, CookMichael, G. FitzGeraldRoss, Swofford
In silico studies of available transcript sequence data have found that up to 24% of human protein coding loci also encode cis-NAT s [8, 9]. However, antisense transcripts tend to be poly(A) negative and nuclear localized [10]. If this is true, the abundance of NAT s (cis and trans...
(<200 nt) were filtered. Transcript models that did not pass an optimized read coverage threshold in both biological replicates were removed (“Methods,” Additional file2: Figure S1A). We then analyzed the protein coding potential of the remaining transcripts using three computational tools: CPC...
The years following publication of the draft human genome sequence brought about a dramatic change in our understanding of the organization and expression of genetic information. Discoveries of new classes of RNA without protein coding potential and larg
In cases where there is perfect complementarity between miRNA and mRNA, transcript degradation occurs [114]. Various roles of these small regulatory RNAs in cancer have been characterized. Upregulation or downregulation of the RNA molecules which function as oncogenes and tumor suppressors, have been...
The discovery of drivers of cancer has traditionally focused on protein-coding genes1–4. Here we present analyses of driver point mutations and structural variants in non-coding regions across 2,658 genomes from the Pan-Cancer Analysis of Whole Gen