We modified a PAC containing the whole zebrafish scl locus, inserting GFP into the first coding exon of scl. In germline-transgenic zebrafish generated using this construct, GFP expression completely recapitula
Polypurine sequences within a downstream exon function as a splicing enhancer. Mol. Cell. Biol. 14, 1347–1354 (1994). CAS PubMed PubMed Central Google Scholar Carlo, T., Sterner, D. A. & Berget, S. M. An intron splicing enhancer containing a G-rich repeat facilitates inclusion of ...
However, the method of tag insertion we used also disrupts coding regions in the last exon of the proteins analyzed, and some proteins that depend on their C-terminal amino acids may be mislocalized. Thus, failure of a protein to localize to the AIS after endogenous gene tagging should not...
GFP expression cassettes were inserted in the first coding exon at the translation initiation site of the gene of interest using a procedure developed by Yang et al. (1997). BAC Transgenic Mice Circular BAC DNAs were injected into the fertilized eggs of the C57BL/6 strain at a concentration...
Among other new findings, we show that the central proteome is encoded by exon-rich genes, indicating an increased regulatory flexibility through alternative splicing to adapt to multiple environments, and that the protein interaction network linking the central proteome is very efficient for ...
RhCMV 68-1-ATCC only has the first exon of UL130, and is missing the second exon which has been termed rh157.4 (Oxford et al., 2008). Although not reported in the initial publications (Marsh et al., 2011, Rivailler et al., 2006), analysis of both RhCMV 180.92 and CynoCMV ...
The sites flanking Iα promoter/exon targeted by the gRNAs are indicated with arrows. The Eµ/Iµ enhancer/promoter between the variable and the constant regions, and the 3′RR super-enhancer downstream of the locus are shown. (B) Flow cytometry analysis of Iα-deleted clones. The 8 ...
at the Greenwood Genetics Center from a previous study, we were able to amplify by PCR and screen by Sanger sequencing the coding regions (including intron/exon boundaries) of the following genes: SHANK3 (22 exons), MAPK8IP2/IB2 (13 exons), RABL2B (8 exons), hsa-miR-1249 (1 exon)...
The exon/intron structure of IL-19 is similar to that of the human IL-10 gene, comprising five exons and four introns within the coding region of the IL-19 cDNA. There are at least two distinct IL-19 mRNA species that differ in their 5'-sequences, suggesting the existence of an ...
Genetic screening was performed, and the boy and his sister exhibited two novel compound heterozygous mutations in the branched chain keto acid dehydrogenase E1 subunit beta (BCKDHB) gene: A substitution from guanine to adenine in the coding region at position 1,076 (c.1,076G>A) in exon 10...