Non-canonical Amino Acid Incorporation into 2,5-Diketopiperazines by Cyclodipeptide SynthasesCyclodipeptide synthasesDiketopiperazinesNatural product engineeringNon-canonical amino acidBiosynthesisThe manipulation of natural product biosynthetic pathways is a powerful means of expanding the chemical diversity of ...
non-canonical amino acid, synthetic biosafety, post-translational modificationThe incorporation of noncanonical amino acids (ncAAs) has been extensively studied because of its broad applicability. In the past decades, various in vitro and in vivo ncAA incorporation approaches have been developed to ...
Residue-specific methods have since been applied to identify de novo protein synthesis in a variety of contexts. Dieterich et al. introduced the bioorthogonal non-canonical amino acid tagging (BONCAT) strategy for selective analysis of de novo protein synthesis with high temporal resolution [22,72]....
Whereas nature relies on a small number of amino acid structures, chemists desire access to a vast range of structurally diverse analogues1,2,3. The selective modification of amino acid side-chain residues represents an efficient strategy to access non-canonical derivatives of value in chemistry ...
(Fig.5)29. We suspect that a limiting factor here is the translocation of BzHis-tRNA LeuCAGto the ribosome by EF-Tu-GTP. The EF-Tu-AA-tRNA-GTP ternary complex derives its binding affinity from both the amino acid and the tRNA body50,51,52,53, and this can affect the incorporation ...
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(2005) American Chemical Society Meeting: Unnatural amino acid could prove boon for protein therapeutics. Science 308: pp. 44 Mehl, R.A., Anderson, J.C., Santoro, S.W. (2003) Generation of a Bacterium with a 21 Amino Acid Genetic Code. J Am Chem Soc 125: pp. 935-939 CrossRef ...
This dissertation primarily describes investigations of the mechanisms by which pentameric ligand-gated ion channels (pLGICs) activate ("gating") using canonical and non-canonical amino acid mutagenesis. Chapter 1 provides an introduction to the systems being studied, their physiological roles, and the...
United StatesBioOrthogonal Non-Canonical Amino acid Tagging (BONCAT) is a powerful tool fortracking protein synthesis on the level of single cells within communities and wholeorganisms. A basic premise of BONCAT is that the non-canonical amino acids (NCAA)used to track translational activity do not...
From left to right, lane 1 is the protein marker; lane 2 is wild-type full-length MDH; lane 3 is MDH with 140 position mutated to a UAG stop codon and without any noncanonical amino acid supplied in the growth media; lane 4 is MDH with 140 position mutated to a UAG stop codon and...