We anticipate that the studies highlighted in this article, coupled with the continuing advancements in genetic code expansion technology, will promote the widespread use of noncanonical amino acids in biocatalysis research in the coming years.Copyright 2020 Elsevier Ltd. All rights reserved....
Aromatic noncanonical amino acids that are made using biocatalysis. Single step, enzyme synthesis makes hundreds of tryptophan and other amino acid analogs!
For the last two decades, synthetic biologists have been able to unlock and expand the genetic code, generating proteins with unique properties through the incorporation of noncanonical amino acids (ncAAs). These evolved biomaterials have shown great potential for applications in industrial biocatalysis,...
The use of Genetic Code Expansion technology to enable the site-specific incorporation of noncanonical amino acids (ncAAs) into proteins in living cells has transformed our ability to study biological processes and provided the exciting potential to develop modern medicines1,2,3,4,5,6,7,8. The...
noncanonical amino acidC-H hydroxylationbiocatalysisnonheme dioxygenasenatural productsModified amino acids are important building blocks in both natural biomolecules... More by Hans Renata,emailprotected,* Email for H.R.: emailprotected,... - 《Acs Catalysis》 被引量: 0发表: 2023年 Selective identi...
In general, conventional enzyme engineering utilizes 20 canonical amino acids to alter and improve the functional properties of proteins such as stability, and activity. In this study, we utilized the noncanonical amino acid incorporation technique to enhance the functional properties of ω-transaminase ...
Noncanonical cofactor biomimetics (NCBs) such as nicotinamide mononucleotide (NMN+) provide enhanced scalability for biomanufacturing. However, engineering enzymes to accept NCBs is difficult. Here, we establish a growth selection platform to evolve enzy
biocatalysisenzyme expressionnoncanonical amino acidsprotein engineeringpseudomonas fluorescens esteraseProtein design is limited by the diversity of functional groups provided by the canonical protein ?building blocks". Incorporating noncanonical amino acids (ncAAs) into enzymes enables a dramatic expansion of ...
biocatalysisimmobilizationthermostabilitytransaminaseIn general, conventional enzyme engineering utilizes 20 canonical amino acids to alter and improve the functional properties of proteins such as stability, and activity. In this study, we utilized the noncanonical amino acid incorporation technique to enhance ...
amino acidsbiocatalysisenzymesheterocycleslyasesCarbon‐nitrogen (C㎞) lyases are enzymes that normally catalyze the cleavage of C㎞ bonds. Reversing this reaction towards carbon–nitrogen bond formation can be a powerful approach to prepare valuable compounds that could find applications in everyday life...