Genetically encoded noncanonical amino acids have provided new tools to probe complex enzyme mechanisms, improve biocatalyst activity and stability, and most ambitiously to design enzymes with new catalytic mechanisms that would be difficult to access within the constraints of the genetic code. We ...
The standard proteinogenic amino acids grant access to a myriad of chemistries that harmonize to create life. Outside of these twenty canonical protein building blocks are countless noncanonical amino acids (ncAAs), either found in nature or created by man. Interest in ncAAs has grown as researc...
H. Nitroalkanes as versatile nucleophiles for enzymatic synthesis of noncanonical amino acids. ACS Catal. 9, 8726–8730 (2019). Article CAS PubMed PubMed Central Google Scholar Werner, E. R., Blau, N. & Thöny, B. Tetrahydrobiopterin: biochemistry and pathophysiology. Biochem J. 438, 397...
a, The α-amines of l-α-amino acids are recognized differently by MmPylRS (left)37 and MjTyrRS (right)36. b, Analogues of l-BocK 1 evaluated as substrates for MaPylRS. c, RNAse A assay used to detect the acylation of Ma-tRNAPyl with the BocK analogues shown in b. d, LC–HRMS...
The efficiency, selectivity and sustainability benefits offered by enzymes are enticing chemists to consider biocatalytic transformations to complement or even supplant more traditional synthetic routes. Increasing demands for efficient and versatile synthetic methods, combined with powerful new discovery and eng...
An evolved pyrrolysyl-tRNA synthetase with polysubstrate specificity expands the toolbox for engineering enzymes with incorporation of noncanonical amino acids Ke Liu, Ling Jiang, Shuang Ma, Zhongdi Song, Lun Wang… Article:92 Download PDF (6057KB) View Article Research Mechanistic insights into...
Figure 1. Metabolic Functions of Metabolic Enzymes with Moonlighting Functions Metabolic pathways regulated by metabolic enzymes, which process moonlighting functions, are shown. Table 1. Moonlighting Functions of Metabolic Enzymes Metabolic EnzymesNoncanonical FunctionsMetabolic EnzymesNoncanonical Functions ACLY ...
Partner with us to speed the availability of what you need beyond the noncanonical amino acids currently offered Finding the right building block is a challenge. Finding the exact ones that you need even more so. We can work with your drug design team and key stakeholders to bring forward new...
Humans have∼40 E2s that are involved in the transfer of Ub or Ub-like (Ubl) proteins (e.g., SUMO, NEDD8, etc). All E2s contain a core catalytic domain of roughly 150 amino acids, termed the UBC domain (Figure 1A). This domain adopts an α/β-fold typically with four α-helice...
The addition of mono-ubiquitin or poly-ubiquitin chain to signaling proteins in response to DNA damage signal is thought to be a critical event that facilitates the recognition of DNA damage lesion site, the activation of checkpoint function, termination