Noncanonical amino acids2021年11月15日,北京大学药学院天然药物及仿生药物国家重点实验室刘涛研究员团队在化学生物学领域顶级期刊Nature Chemical Biology (IF=15.0)在线发表了最新研究成果"Genetic code expanded cell-based therapy for treating diabetes in mice".近年来,随着CAR-T细胞的成功上市,利用合成生物学设计的...
For the last two decades, synthetic biologists have been able to unlock and expand the genetic code, generating proteins with unique properties through the incorporation of noncanonical amino acids (ncAAs). These evolved biomaterials have shown great potential for applications in industrial biocatalysis,...
Extending saturation mutagenesis with a range of non-canonical amino acids (ncAA) potentially increases the ability to improve activity and stability simultaneously. Previously, an E. coli transketolase variant (S385Y/D469T/R520Q) was evolved to accept aromatic aldehydes not converted by wild-type....
The structure of noncanonical amino acid (NCAA) side chains allows them to explore conformations inaccessible to canonical amino acids (CAAs). Peptides made of the D-enantiomers of amino acid backbones are resistant to proteolysis. The long term goal of this research is to adapt the current too...
Expansion of the genetic code to noncanonical amino acids (NCAAs) has been limited by the lack of evolutionary pressure for organismal dependence on the NCAA. Linking bacterial survival to an engineered [beta]-lactamase that requires a non-natural tyrosine analog engenders diverse bacteria with a ...
To efficiently prepare homogeneous protein multiconjugates, we design two encodable noncanonical amino acids (ncAAs), 4-(6-(3-azidopropyl)-s-tetrazin-3-yl) phenylalanine (pTAF) and 3-(6-(3-azidopropyl)-s-tetrazin-3-yl) phenylalanine (mTAF), containing mutually ...
The structure of noncanonical amino acid (NCAA) side chains allows them to explore conformations inaccessible to canonical amino acids (CAAs). Peptides made of the D-enantiomers of amino acid backbones are resistant to proteolysis. The long term goal of this research is to adapt the current ...
noncanonical amino acidspost-translational modificationssynthetic biosafetyThe incorporation of noncanonical amino acids (ncAAs) has been extensively studied because of its broad applicability. In the past decades, various in vitro and in vivo ncAA incorporation approaches have been developed to generate ...
Incorporation of noncanonical amino acids (ncAAs) at stop codons provides a site-specific method for introducing unique chemistry into proteins, though often at reduced yields compared to wild-type proteins. A powerful platform for ncAA incorporation supports both the expression and evaluation of ...
The structure of noncanonical amino acid (NCAA) side chains allows them to explore conformations inaccessible to canonical amino acids (CAAs). Peptides made of the D-enantiomers of amino acid backbones are resistant to proteolysis. The long term goal of this research is to adapt the current ...