Non-canonical amino acids in protein polymer design. Polym. Rev. 47, 9-28.Connor RE, Tirrell DA. Non-Canonical Amino Acids in Protein Polymer Design. Polym. Rev. 2007; 47:9-28.Connor, R.E.; Tirrell, D.A. Non-Canonical Amino Acids in Protein Polymer Design. Polym. Rev. 2007, 47,...
Examples of non-canonical amino acids. Chemical structures of amino acids highlighted in this review: methionine (Met), homoallylglycine (Hag), homopropargylglycine (Hpg), azidohomoalanine (Aha) and azidonorleucine (Anl). Azidophenylalanine (Azf), and acetylphenylalanine (Acf) are analogs of pheny...
Amino acids are essential building blocks in biology and chemistry. Whereas nature relies on a small number of amino acid structures, chemists desire access to a vast range of structurally diverse analogues1,2,3. The selective modification of amino acid side-chain residues represents an efficient ...
Protein engineering with non-canonical amino acids (ncAAs) holds great promises for diverse applications, however, there are still limitations in the implementation of this technology at manufacturing scale. The know-how to efficiently produce ncAA-incorporated proteins in a scalable manner is still ve...
Incorporation of non-canonical amino acidsL-menthol propionateDiastereoselectivityMolecular dynamics simulationsIt remains a challenge to improve the diastereopreference of an enzymes when there are multiple chiral centers in the substrate, mainly because the limited understanding of mechanism determining ...
www.nature.com/scientificreports OPEN Expanding the genetic code of Salmonella with non-canonical amino acids received: 02 September 2016 accepted: 29 November 2016 Published: 23 December 2016 Qinglei Gan1, Brent P. Lehman2, Thomas A. Bobik2 & Chenguang Fan1 The diversity of non...
We demonstrate that itRNATy2 can initiate translation in vivo with aromatic nonヽanonical amino acids (ncAAs) bearing diverse sidechains. Although the initial system suffered from low yields, deleting redundant copies of tRNAfMet from the genome afforded an E. coli strain in which the efficiency...
6.Besides, optimization isn’t necessarily the goal, given that the experiments being done to incorporate noncanonical amino acids are exploratory and geared toward applications, not theoretical research. 7.George Church, the prominent geneticist at Harvard University known for his entrepreneurial endeavors...
2F). We also calculated the generating efficiency of source genes, which were defined as the ratio of the number of amino acids detected in the immunopeptidome to the number of amino acids in the source protein. Surprisingly, the efficiency of generating MAPs exhibited the opposite trend and ...
s modified Eagle’s medium (DMEM, HyClone, SH3002202b) supplemented with 15% ESC-qualified fetal bovine serum (FBS, Vistech, SE200-ES), 0.1 mM 2-mercaptoethanol (Sigma, m3148), 1× non-essential amino acids (NEAA, Gibco, 11140050), and 1000 U/mL of LIF (Millipore, ESG1107)....