PathoQuest provides biosafety assays based on next-generation sequencing (NGS) for biologics quality testing. GMP biotesting for biopharmaceuticals. Learn more.
However, the ability to detect these mutations in remission bone marrow specimens is hampered by the limited sensitivity of conventional polymerase chain reaction (PCR)-based assays, which detect approximately only 1 of every 100 (1%) mutant cells. To address this problem, we have developed a ...
1. Solomon JP, Linkov I, Rosado A, et al., NTRK fusion detection across multiple assays and 33,997 cases: diagnostic implications and pitfalls. Mod Pathol. 2020 Jan;33(1):38-46. 2. Desmeules P, Boudreau DK, Bastien N, et a...
Using our recently developed assays for comprehensive profiling of HPV E6/E7 transcripts, we have detected transcriptional activities of 10 high-risk HPV st 展开 年份: 1958 收藏 引用 批量引用 报错 分享 全部来源 求助全文 bases.bireme.br
Due to the often-low copy numbers in foods, sensitive Polymerase Chain Reaction (PCR)- based assays have been developed that target specific re- gions in the genome that can be used for typing [7, 8]. Surveillance plays a crucial part in linking outbreaks and surveillance networks for ...
However, EGFR mutations can easily be detected using PCR-based assays, so the same results could have been obtained using such an approach [3]. However, with the development of novel targeted treatment options in lung cancer, the NGS approach might become more relevant in order to cover all ...
Other techniques used include the hybridisation-based SPF LiPA method, signal-amplification assays (Hybrid Capture 2 and Cervista) and nucleic-acid-based amplification-like microarray, real-time PCR-based methods (COBAS 4800 real-time test) (Kleter et al, 1998; Brink et al, 2007; Abreu et al...
Other techniques used include the hybridisation-based SPF LiPA method, signal-amplification assays (Hybrid Capture 2 and Cervista) and nucleic-acid-based amplification-like microarray, real-time PCR- based methods (COBAS 4800 real-time test) (Kleter et al, 1998; Brink et al, 2007; Abreu et ...
Although DNA barcoding has been largely applied to different food sectors, studies on the traceability and mislabelling of pet foods are still scarce and generally performed on single species-based products using direct Sanger sequencing23,24,25,26or multiple species with qPCR assays9. To the best...
To bring such a complex technology to clinical diagnostic laboratories, limitations and challenges should be recognized when designing and implementing assays for routine clinical use. While most NGS platforms can generate enormous amount of data in a massively parallel manner, the complexities of test ...