DNA-based NGS检测基因融合灵敏度、特异性较高,可满足大部分肿瘤患者需求 一项基于DNA-based NGS panel的大样本回顾性研究分析显示,基于DNA-based NGS检测NTRK融合具有81.1%的总体敏感性和99.9%的特异性,证明了DNA-based NGS对NTRK融合进行检测是可行的...
融合基因DNA和RNA水平上的结构特征决定了融合基因检测,RNA水平远比DNA水平更敏感,更准确,但是样本要求较高。 DNA-based NGS对样本要求相对较低,可联合其他biomarker共同检测,同时DNA层面可检出的罕见融合伴侣、外显子断点融合及基因间融合,但需在RNA或蛋白水平进一步验证。 在临床应用中,全面准确地肿瘤靶基因检测需要临...
结果显示，在DNA-based NGS检测为融合阳性的患者中，RNA-based NGS的检出率为95%（38/40）；在DNA-b...
多项临床研究显示,相较于DNA-based NGS,RNA-based NGS能够多检出8.3%~14.2%的aSVs,通过RNA-based NGS检测,MET剪切变异引起的14号外显子跳跃突变(MET 14跳突)可提升至2.42%。此外,在既往研究中显示,在DNA检测阴性但RNA检测到融合阳性的患者中,约70%~80%患者对靶向治疗响应,可见RNA-based NGS检测到的aSVs与靶...
目前,综合使用二代测序(NGS)进行分子筛选用于非小细胞肺癌(NSCLC)的病理评估被认为是临床实践的标准。对于使用直径1.1 mm冷冻探针取样的肿瘤组织进行NGS的可行性,我们知之甚少。本研究旨在探讨使用直径1.1 m…
Next-generation sequencing (NGS)Species identificationMislabelingSea cucumbers are widely consumed in Asia. This study developed a novel DNA mini-barcode mitochondrial cytochrome c oxidase (COI) of 257 bp to discriminate sea cucumbers species in both raw and processed forms. We designed and compared ...
DNA-based NGS Services Reduced Representation Bisulfite Sequencing (RRBS) Whole Genome Bisulfite Sequencing (WGBS) Chromatin-based NGS Services ChIP-Seq ELISA-based Services Global DNA 5-mC Quantification Global DNA 5-hmC Quantification Global RNA m6A Quantification Global RNA 5-mC Quantification Histone...
(MRD). This approach could provide the capability to adapt clinical trials in the adjuvant setting in order to optimize risk stratification, and we argue that this objective is achievable with current technologies. Herein, we evaluate contemporary next-generation sequencing (NGS) approaches to ctDNA...
The rapid adoption of next-generation sequencing (NGS) (also known as massively parallel sequencing; MPS) techniques has revolutionized the way molecular diagnosis is performed in a clinical diagnostic laboratory. Hundreds to thousands of genes can be analyzed simultaneously, and samples can be ...
ctDNA testing for ERBB2 mutations was performed using two technologies (i.e., ddPCR and NGS with Guardant360); in 784 cases both analyses were performed. In addition, in 77 cases, either a fresh recurrent tumor biopsy or an archival FFPE recurrent tumor biopsy was used for tissue sequencing...