These pts may benefit from earlier NGS testing, which would have opened up additional therapies earlier on in the course of their disease, when their PS was also likely to be more optimal. Further work is necessary to determine if early NGS testing makes a significant clinical impact on ...
PCR conditions and sequencing protocols can be found in Suppl. material 1: ESM2. NGS bioinformatics NodC sequences were joined, and sequences < 150bp in length or with ambiguous base calls were removed. Sequences were quality filtered using a maximum expected error threshold of 1.0 and de...
For tumors that rarely harbor NTRK fusions, front-line NGS-based approaches or a two-step approach with IHC followed by sequencing tests are indicated32. IHC examines the expression of TRK proteins but does not directly detect NTRK fusions34,35. It can be performed through different antibodies...
With the continuous development of high-throughput sequencing technology, several rice thermal-responsive miRNAs have been identified through reverse genetics, and potential downstream target genes have been predicted. Li et al. (2014) constructed a small RNA library of young panicles of the heat-tole...
Whole exome sequencing (WES) has traditionally been used to assess TMB, but high cost limits its widespread use. However, alterna- tive methods such as next-generation sequencing (NGS) effectively stratify patients by likelihood of response [24]. Two NGS panels, the Oncomine Tumor Mutation Load...
Next-generation sequencing (NGS) is a technique used to analyze and sequence nucleic acids (DNA/RNA) and has made a big advancement in molecular biology. It is a fast, cheap, and scalable technique that allowed for investigating a wide range of biological research and studies that were previou...
Next-generation sequencing (NGS)–based MRD assessment has gained increasing interest. However, some challenges (including how to deal with markers for clonal hematopoiesis) remain, so this method is not established in routine practice.14, 15, 16 Following the ELN recommendations, we use RT-qPCR ...
Computational workflows are often notjustcomputational workflows. Most interface with Library Information Management Systems (LIMS), Next-generation Sequencing (NGS) instruments, perform complex input validations, and coordinate processing between on-prem and cloud services. Many developers find that writing ...
Many Next-Gen Sequencing (NGS) library preparations still require resolving the samples using agarose gels to separate out fragments before sequencing. However, recovering DNA from gel excisions can be challenging with many researchers struggling to recover more than 50% yield from the original input...
Furthermore, among patients with hematologic response, gilteritinib induced a molecular response (variant allele frequency [VAF] ≤10−2) using a next-generation sequencing (NGS)-based FLT3-ITD–specific assay in 25% of evaluable cases. FLT3-ITD detection by capillary electrophoresis, as used ...