We found that the p53-R273 contact mutant, but not the p53-R175 conformational mutant, promotes cancer cell survival and resistance to anoikis of cancer cells. Underlying these activities is the ability of p53-R273H mutant to suppress BMF expression in a way that is dependent on PI3K/AKT ...
We sought to examine the role of mutant p53 in pre-mRNA processing, especially in IPA regulation in lung cancer.Methods: We established stable cell lines transfected with mutant p53 (R273H, R175H, H179Q, C238Y, and C242F) or vector control using the NSCLC cell line H1299 with a ...
AH1299 cells, stably expressing mutant p53 R273H or an empty vector (EV) (PCB6) were transfected with GFP or GFP-RCP and cell lysates were immunoprecipitated with GFP. P-gp and GFP expression was analysed by western blot.BCell lysates of H1299 cells stably expressing mutant p53 R273H or ...
The presence of mutant p53 in KPC+sh.Ctrl cells enhanced invasiveness, which was significantly abrogated upon p53 knockdown (Figure 1B). The ability of mutant p53 to drive cell invasion was also evident following enforced expression of p53R175H and p53R273H, two mutants frequently found in ...
e Co-immunoprecipitation of exogenous TP53R273H-FLAG with MYC-BCAR1 in the presence or absence of c-SRCY530F. Lysates of H1299 cells transfected with the indicated plasmids were immunoprecipitated with anti-MYC antibody and analysed by Western blot. An empty vector or MYC tag-containing plasmid...
To compare different p53 mutants in an isogenic cellular context, we co-transfected TBK1 with different p53 mutants (P142L, P152Q, A161V, C174Y, R175H, R248W, R249S, R273H, R280K, and wild-type [WT]p53) into H1299 cells. We observed that the different mutants interacted with ...
First, to generate stable P53 knockdown cell lines, lentiviral transduction particles from TRC-pLKO-U6 shP53 were infected into MCF10A. To generate mutant P53 producing MCF10A, pcDNA mutant P53 was transfected using lipofectamin (Invitrogen) into stable P53 knockdown cell lines. Glucose uptake assay...
The 3D structure of mutant p53 proteins after TP53 amino acid residues were mutated as p.R175H, p.R273H, p.R282W, or p.V274F. These mutant p53 protein hotspots were revealed in the DNA binding domain to affect its hydrogen bonding conformation....
Many cancers express mutant p53 proteins that have lost wild-type tumor suppressor activity and, in many cases, have acquired oncogenic functions that can contribute to tumor progression. These activities of mutant p53 reflect interactions with several o
transfected with the indicated plasmids were immunoprecipitated with anti-FLAG antibody (c) or with anti-TP53 antibody (d), and analysed by Western blot. A FLAG tag-containing vector was used as a negative control for FLAG-BCAR1 in (c).e–gCo-immunoprecipitation of endogenous TP53R273Hand ...