(2001). Mdm2 mutant defective in binding p300 promotes ubiquitination but not degradation of p53. Evidence for the role of p300 in integrating ubiquitination and proteolysis. J. Biol. Chem. 276, 29695-29701.Zhu,
Wild-type p53 is stabilized and accumulates in the nucleus of DNA damaged cells. The effect of stabilizing p53 is to inhibit cell growth, either through a G1 cell cycle arrest or apoptotic cell death. MDM2 can inhibit p53 activity, in part, by promoting its rapid degradation through the ...
UBE2O promotes poly-ubiquitination and degradation of Mxi1 Given that UBE2O is an E2/E3 hybrid ubiquitin-protein ligase and has been reported to mediate ubiquitination of several proteins, we therefore speculated that Mxi1 might be a ubiquitination substrate of UBE2O. As shown in Fig. 2a, ...
1, B). To see whether the ubiquitination mediated by CHIP will lead to the degradation of eIF2α, cells were transfected with different amounts of CHIP and protein levels of endogenous eIF2α were detected with an anti-eIF2α antibody. We found that the protein levels of eIF2α decreased...
Wild-type p53 is stabilized and accumulates in the nucleus of DNA damaged cells. The effect of stabilizing p53 is to inhibit cell growth, either through a G1 cell cycle arrest or apoptotic cell death. MDM2 can inhibit p53 activity, in part, by promoting its rapid degradation through the ubi...
Further studies demonstrated that Tim-AIII has therapeutic effect on NSCLC by binding to its target HSP90, which promotes ubiquitination and degradation of GPX4 ultimately induces NSCLC cells ferroptosis. These findings highlight the role of Tim-AIII in the treatment of NSCLC and reveal a ...
org/10.1016/j.ccr.2010.08.010 Zhang S, Zhou L, Hong B, van den Heuvel AP, Prabhu VV, Warfel NA et al (2015) Small-molecule NSC59984 restores p53 pathway signaling and antitumor effects against colorectal cancer via p73 activation and degradation of mutant p53....
Importantly, theSpopconditional prostate knockout mice have been generated and display dramatically increased AKT kinase activity [27]. Meanwhile, another study focused on SPOP-mediated BRDs degradation also revealed that depletedSPOPcould induce AKT kinase activity potentially by upregulating BRDs downstream...
Here, we show that specific interaction of wild-type Vpu protein with SCF complex leads to inhibition of ubiquitination and proteasomal degradation of p53 protein in a β-TrcP–dependent manner. Successful interaction of SCFβ-TrcP complex with β-TrcP binding motif (DS52GNES56) present in Vpu ...
CHIP is a chaperone associated ubiquitin ligase that has been shown to exhibit tumor suppressive effects through catalyzing the ubiquitination and subsequent degradation of several oncogenic proteins such ErbB2, c-myc, mutant p53, and NF-κB [126], [127], [128], [129]. CHIP has also been ...