Multi-particle cryo-EM refinement with M visualizes ribosome-antibiotic complex at 3.5 in cellsCryo-electron microscopy (cryo-EM) enables macromolecular structure determination in vitro and inside cells. In addition to aligning individual particles, accurate registration of sample motion and three-...
Cryo-electron microscopy (cryo-EM) enables macromolecular structure determination in vitro and inside cells. In addition to aligning individual particles, accurate registration of sample motion and three-dimensional deformation during exposures are cruci
Multi-particle cryo-EM refinement with M visualizes ribosome-antibiotic complex at 3.5 in cells 来自 NCBI 喜欢 0 阅读量: 42 作者:D Tegunov,X Liang,C Dienemann,P Cramer,J Mahamid 摘要: Cryo-electron microscopy (cryo-EM) enables macromolecular structure determination in vitro and inside cells....
The ROME (Refinement and Optimization via Machine lEarning for cryo-EM) software package is a parallel computing software system dedicated for high-resolution cryo-EM structure determination and data analysis, which implements advanced machine learning a
Cryo-EM Foundations Image Formation Processing Data in cryoSPARC Get Started with CryoSPARC: Introductory Tutorial (v4.0+) Tutorial Videos All Job Types in CryoSPARC CryoSPARC Tools Data Processing Tutorials Webinar Recordings Real-time processing in cryoSPARC Live ...
This manuscript describes the cryo-EM structure of SARS-CoV-2 spike ectodomain trimer in two different conformational states, closed and partially open (one SBdomain open), providing new insights for the design of vaccines and inhibitors of viral entry. They found that the SARSCoV-2 S glycoprote...
Here, to explore the activation mechanism of Monalysin, we determined three crystal structures of Monalysin (pro and cleaved forms and an inactive mutant) as well as the cryo-EM reconstruction of the pro-Monalysin particle. Coupled with studies in solution using light scattering (SEC/MALS/UV/...
Here we describe M , a software tool that establishes a reference-based, multi-particle refinement framework for cryo-EM data and improves the results of structure determination. M provides a unified optimization framework for both in vitro frame series and in situ tomographic tilt series data. ...
To further understand the role of SHM, we investigated the interaction between mAb 23 and a D614G-derived prefusion-stabilized spike by cryo-EM (Fig.5band Supplementary Fig.5). The solved structure revealed the capacity of mAb 23 to bind with the RBD in both “up” and “down” conformati...
Fig. 1: Cryo-EM structures of reconstituted mouse RS head-neck complex. aCoomassie-stained PAGE of the reconstituted RS head-neck complex. This was repeated three times independently with similar results. Source data are provided as a Source Data file.bRepresentative reference-free 2D class averag...