转录组测序的研究对象为特定细胞在某一功能状态下所能转录出来的所有RNA的总和,主要包括 mRNA和非编码RNA 。转录组研究是基因功能及结构研究的基础和出发点,通过新一代高通量测序,能够全面快速地获得某一物种特定组织或器官在某一状态下的几乎所有转录本序列信息,已广泛应用于基础研究、临床诊断和药物研发等领域。爱默...
在这里,我们提出了一种多路复用单细胞mRNA测序方法,用于分析小鼠胚胎组织中的基因表达。基于滴滴的单细胞mRNA测序(scRNA-Seq)方法与多路复用策略相结合,可以同时分析来自多个样品的单个细胞,从而显著降低试剂成本并最大限度地减少实验批次效应。 Abstract 单细胞mRNA测序在过去几年中取得了显著进展,已成为发育生物学领域的...
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mRNASequencingSamplePreparation3.FragmentationofmRNA ThemRNAisfragmentedintosmallpiecesusingdivalentcationsunderelevatedtemperature.5’-UCGGAAGCUGAAGUGAUCAGGGUUCAAUAAAAAAAAAAAAA-3’mRNA Randomfragmentation 5’3’5’5’5’3’5’5’5’3’5’3’5’3’5’3’3’5’3’5’3’5’3’5’3’Add...
Qiwen Yanghttps://ror.org/0051rme32grid.144022.10000 0004 1760 4150Key Laboratory of Animal Genetics, Breeding and Reproduction of Shaanxi Province, College of Animal Science and TechnologyNorthwest A&F University Yangling 712100 Xianyang Shaanxi Province ChinaFuwen Wang...
锐博生物>产品与服务索引>高通量测序>mRNA测序 转录组研究是基因功能及结构研究的基础和出发点,通过第二代高通量测序,能够全面快速地获得某一物种特定组织或器官在某一状态下的几乎所有转录本序列信息,从而准确地分析基因表达差异、发现新转录本、基因结构变异、基因融合、RNA编辑等生命科学的重要问题,已广泛应用于基础...
et al. QuantSeq 3′ mRNA sequencing for RNA quantification. Nat Methods 11, i–iii (2014). https://doi.org/10.1038/nmeth.f.376 Download citation Published25 November 2014 Issue DateDecember 2014 DOIhttps://doi.org/10.1038/nmeth.f.376 Subjects Next-generation sequencing RNA sequencing This ...
RNA-Sequencing (RNA-Seq) of peripheral blood can be a valuable source of information for investigating the status and mechanism of diseases. However, blood contains 50–80% unwanted hemoglobin (Hb) transcripts. Lexogen’s QuantSeq mRNA-Seq-Kit for Illumina RNA-Seq features a ‘Globin Block’ ...
Screening for quantitative real-time PCR reference genes with high stable expression using the mRNA-sequencing data for pear 来自 Semantic Scholar 喜欢 0 阅读量: 108 作者:Y Wang,M Dai,D Cai,Z Shi 摘要: Housekeeping genes were considered to have a constant expression without spatio-temporal ...
2.3. mRNA-Sequencing RNA purity was analyzed using a NanoDrop 8000 spectrophotometer (Thermo Fisher Scientific), and the total RNA integrity was determined according to the RNA integrity number (RIN) using a 2100 Bioanalyzer instrument (Agilent Technologies, Santa Clara, CA, USA). A RIN exceeding...