转录组测序的研究对象为特定细胞在某一功能状态下所能转录出来的所有RNA的总和,主要包括 mRNA和非编码RNA 。转录组研究是基因功能及结构研究的基础和出发点,通过新一代高通量测序,能够全面快速地获得某一物种特定组织或器官在某一状态下的几乎所有转录本序列信息,已广泛应用于基础研究、临床诊断和药物研发等领域。爱默...
Here, we present full-length poly(A) and mRNA sequencing (FLAM-seq), a rapid and simple method for high-quality sequencing of entire mRNAs. We report a complementary DNA library preparation method coupled to single-molecule sequencing to perform FLAM-seq. Using human cell lines, brain organoid...
44 cells were captured as a single isolate on a C1 array chip for mRNA sequencing (17–25 μm) as determined by microscopic examination, and 34 passed the required criteria for cDNA quantity and quality
Zebrafish mRNA sequencing deciphers novelties in transcriptome dynamics during maternal to zygotic transition. Genome Res 21:1328–1338. : 10.1101/gr.116012.110Aanes H , Winata CL , Lin CH , Chen JP , Srinivasan KG , Lee SG , Lim AY , Hajan HS , Collas P , Bourque ...
锐博生物>产品与服务索引>高通量测序>mRNA测序 转录组研究是基因功能及结构研究的基础和出发点,通过第二代高通量测序,能够全面快速地获得某一物种特定组织或器官在某一状态下的几乎所有转录本序列信息,从而准确地分析基因表达差异、发现新转录本、基因结构变异、基因融合、RNA编辑等生命科学的重要问题,已广泛应用于基础...
mRNA Sequencing Sample Preparation 2. mRNA Purification from Total RNA mRNA is isolated from total RNA by binding the mRNA to a magnetic oligo(dT) bead. mRNA has a polyA tail and will bind to the oligo(dT) bead. mRNA 5’-UCGGAAGCUGAAGUGAUCAGGGUUCAAUAAAAAAAAAAAAA-3’ Magnetic Oligo (...
genome-wide identification of long non-coding rna and mrna profiling using rna sequencing in subjects with sensitive skin.[2017][oncotarget][10.186 热度: Stemcelltranscriptomeprofilingviamassive-scale mRNAsequencing NicoleCloonan1,4,AlistairRRForrest1,3,4,GabrielKolle1,4,BrookeBAGardiner1,GeoffreyJFaulkn...
mRNA sequencing. The Encode RNA-PET dataset captures the connections between 5′ and 3′ ends of transcripts. These data were used for the validation of the combinations of TSSs and PASs in the transcript structures identified by full-length mRNA sequencing in MCF-7 cells. However, it is ...
RNA-Sequencing (RNA-Seq) of peripheral blood can be a valuable source of information for investigating the status and mechanism of diseases. However, blood contains 50–80% unwanted hemoglobin (Hb) transcripts. Lexogen’s QuantSeq mRNA-Seq-Kit for Illumina RNA-Seq features a ‘Globin Block’ ...
Other challenges in the preparation of single-cell transcriptome sequencing samples, such as the lengthy time for single-cell lysis and poor mRNA capture efficiency, will affect the quality of the sequencing library and further affect the accuracy and sensitivity of the sequencing results. To evaluate...