❝ 基因融合的鉴定,可以基于全基因组测序数据(whole-genome sequencing,WGS),也可以利用转录组测序数据(RNA-seq),或者二种技术结合起来更好。「WGS鉴定出的基因融合」,基本能确定是由于基因组层面发生某种变异而引起的,但如果没有转录组测序数据,就无法准确判断融合后产生的新基因是否能够表达,或表达量的高低。而「...
参考文献: 1. Garg V, Khan A W, Kudapa H, et al. Integrated transcriptome, small RNA and degradome sequencing approaches provide insights into Ascochyta blight resistance in chickpea[J]. Plant Biotechnology Journal, 2019, 17(5): 914-931....
RNA sequencing is widely used in the analysis of cellular gene expression, where it can determine the sequence identity and quantitative abundance of mRNAs. Additional RNA sequencing methods have been developed to measure polyadenylation, nucleoside modifications and RNA secondary structures13,14,15. Howe...
T cell assays Peptide sequencing or Mass Spectrometry analysis 肿瘤抗原的识别机制 PAMPs (Pathogen-associated molecular pattern) 病原识别的机制 PRRs (Pattern recognition receptors) 受体4的识别机制 mRNA疫苗的免疫递程机理 树突状细胞(DC)介导的免疫反应 肿瘤mRNA的制备过程 目前的mRNA疫苗纳米脂质体递送系统和...
data analysis, F.T. and M.A.S. contributed to the cDNA sample preparation, E.N., N.X. and Y.W. constructed libraries, C.L. and J.B. contributed to the library sequencing, F.T., E.N. and K.L. contributed to experimental validation, F.T., K.L. and M.A.S. wrote ...
1. Liu, Y., et al., Poly(A) inclusive RNA isoform sequencing (PAIso-seq) reveals wide-spread non-adenosine residues within RNA poly(A) tails.Nat Commun, 2019. 10(1): p. 5292. 2. Liu, Y., et al., Transcr...
/master/DEG_scripts 从零开始学转录组(7):差异基因表达分析从零开始学转录组(8):富集分析RNA-seq项目设计:生物学重复和单个样本测序量对结果的影响 clusterProfiler参考文档 差异基因分析 文献:Efficient experimental design and analysis strategies for the detection of differential expression using RNA-Sequencing...
A Comparison of mRNA Sequencing (RNA-Seq) Library Preparation Methods for Transcriptome Analysisdoi:10.21203/rs.3.rs-1088423/v1Hiroki UraSumihito TogiYo Niida
single cell RNA 3'-end sequencing 我们在单细胞3' RNA测序中,很重要的工作就是衡量不同基因的表达情况,但是我们是用CPM还是RPKM还是FPKM呢?实际上,你只能并且只会用到CPM,试想一下,3'端测序难道不是测到一个read就是相当于一个mRNA吗?这个数量和基因长度有关系吗?显然没有关系,基因再长,3'端测序终究只能...
1. Hrdlickova R, Toloue M, Tian B (2017) RNA-Seq methods for transcriptome analysis. Wiley Interdiscip Rev RNA 8(1). 2. Garalde DR, Snell EA, Jachimowicz D et al. (2018) Highly parallel direct RNA sequencing on an array of nanopores. Nat Methods 15(3):201–206. ...