Furthermore, we have not found changes in cell population abundance related to the SARS + /F + group, possibly due to the poor quality of CIBERSORTx deconvolution based on our bulk mRNA sequencing data and Dirichlet regression that does not characterize the impact of outliers. Ideally...
(5个基因) 实验验证:实时荧光定量qPCR和HPA数据库证实了LUAD组织中特征基因(5个基因)的mRNA和蛋白质表达模式。
Integrated analysis of single-cell and bulk RNA sequencing data reveals immune-related lncRNA-mRNA prognostic signature in triple-negative breast cancer. Genes Dis. 2023 May 10;11(2):571-574. doi: 10.1016/j.gendis.2023.04.006. PMID: 37692501; PMCID: PMC10491898....
前言 今天给大家分享的文献是2024年3月16日发表在“Journal of Translational Medicine”的一篇将干湿结合经典的文章,题目是《Single-cell transcriptomic sequencing data reveal aberrant DNA methylation in SMAD3 promoter region in tumor-associated fibroblasts affecting molecular mechanism of radiosensitivity in non-s...
1、Tang Fuchou, Barbacioru C, Wang Y, et al. mRNA-Seq whole-transcriptome analysis of a single cell. Nature Methods. 6(5):377-82(2009) 2、Tang, X., Huang, Y., Lei, J. et al. The single-cell sequencing: new developments and medical applications. Cell Biosci 9, 53 (2019) ...
对illumina数据进行处理,利用 RNA-Seq 发现新的 RNA 变体和剪接位点,或量化 mRNA 以进行基因表达分析等。对两组或多组样本的转录组数据,通过差异表达分析和对所发现的差异表达基因集合进行功能富集分析以推断生物学功能。 数据准备: 数据下载: Human genome(GRCh38/hg3):Index of /goldenPath/hg38/chromosomes (...
SequencingUMIDuring the course of an immune response to a virus such as influenza, B cells undergo activation, clonal expansion, isotype switching, and somatic hypermutation (SHM). Members of an antigen-experienced B-cell clone can have different sequence features including SHM......
GO/KEGG function enrichment analysis, Gene set enrichment analysis (GSEA), and Gene set variation analysis (GSVA) were conducted based on hub genes, and mRNA-miRNA, mRNA-TF, and mRNA-drug networks of hub genes were constructed. Single-cell RNA sequencing (scRNA-seq) was used to reveal ...
RNA-sequencing (RNA-seq) has become an increasingly cost-effective technique for molecular profiling and immune characterization of tumors. In the past decade, many computational tools have been developed to characterize tumor immunity from gene expression data. However, the analysis of large-scale RNA...
Spatial transcriptome sequencing data (GSE230282) 基因集选择 NE gene sets from differentially expressed analyses between NEPC and prostatic adenocarcinoma HP_NE_neoplasm gene from MSigDB database (http://www.gsea-msigdb.org/) 生信分析方法 根据文章的分析流程提取所有的分析内容,整理出来就21个分析条目,每...