3.Yoder-Himes DR, Chain PSG, Zhu Y, Wurtzel O, Rubin E, Tiedje JM, Sorek R. Mapping the Burkholderia cenocepacia niche response via high-throughput sequencing. Proc Natl Acad Sci USA, 2009, 106(10): 3976–3981 4.Stewart FJ, Ottesen EA, DeLong EF. Development and quantitative analyses o...
3.Yoder-Himes DR, Chain PSG, Zhu Y, Wurtzel O, Rubin E, Tiedje JM, Sorek R. Mapping the Burkholderia cenocepacia niche response via high-throughput sequencing. Proc Natl Acad Sci USA, 2009, 106(10): 3976–3981 4.Stewart FJ, Ottesen EA, DeLong EF. Development and quantitative analyses o...
3.Yoder-Himes DR, Chain PSG, Zhu Y, Wurtzel O, Rubin E, Tiedje JM, Sorek R. Mapping the Burkholderia cenocepacia niche response via high-throughput sequencing. Proc Natl Acad Sci USA, 2009, 106(10): 3976–3981 4.Stewart FJ, Ottesen EA, DeLong EF. Development and quantitative analyses o...
肿瘤新抗原的预测和验证平台 NGS Sequencing Sample:Fresh/Frozen/FFPE/Celllines(tumor purity,disease stage,site) Sequencing protocol:WES+RNAseq,depth,coverage,read length Variant calling Data quality:PF>80%,Q30>80%,Unique mapping rate >98% Type:SNV,Indel,gene-fusion,CNV? RNASeq:variant expression ...
We developed a massive-scale RNA sequencing protocol, short quantitative random RNA libraries or SQRL, to survey the complexity, dynamics and sequence content of transcriptomes in a near-complete fashion. This method generates directional, random-primed, linear cDNA libraries that are optimized for ...
The gene is synthesized, cloned into a vector, and verified by Sanger sequencing. The plasmid DNA is then amplified, target mRNA sequence linearized, and used as a template for in vitro transcription.Custom mRNA Synthesis Applications Therapeutic Development: Treat diseases of protein expression ...
201,202 TCGA and other deep sequencing studies have also revealed the complexity, heterogeneity, and lability of mutations in tumors.203–208 Among the most relevant findings of TCGA has been the confirmation that mutation of proteins in the tyrosine kinase signaling pathway is common in human ...
Single cell mRNA sequencing technologies have transformed our understanding of cellular heterogeneity and identity. For sensitive discovery or clinical marker estimation where high transcript capture per cell is needed only plate-based techniques current
Sahin and colleagues have pioneered the use of individualized neoepitope mRNA cancer vaccines121. They use high-throughput sequencing to identify every unique somatic mutation of an individual patient's tumour sample, termed the mutanome. This enables the rational design of neoepitope cancer vaccines in...
sequencingChromium 10× 3′ V2 protocol is a 3′ end counting single-cell mRNA sequencing protocol that allows to process and sequence RNA from thousands of cells in parallel. Chromium10× by 10× Genomics is an...doi:10.1007/978-1-4939-9240-9_7Marco De Simone...