We then consider key points for the clinical translation of lipid nanoparticle–mRNA formulations, including good manufacturing practice, stability, storage and safety, and highlight preclinical and clinical studies of lipid nanoparticle–mRNA therapeutics for infectious diseases, cancer and genetic disorders...
解冻后的LNP-RNA系统可以在4°C下保存不超过7天。 Optimization of storage conditions for lipid nanoparticle-formulated self-replicating RNA vaccines
Optimization of storage conditions for lipid nanoparticle-formulated self-replicating RNA vaccines. J Control Release. 2023 Jan;353:241-253. [2]. Henderson MI, Eygeris Y, Jozic A, Herrera M, Sahay G. Leveraging Biological Buffers for Efficient Messenger RNA Delivery via Lipid Nanoparticles. Mol ...
作为新冠疫苗的载体,mRNA 脂质纳米颗粒(LNP,Lipid Nanoparticle)已经获得了巨大的成功。LNP 组分的选择,特别是可离子化的脂质分子能够有效地将转录 COVID-19 病毒刺突蛋白的 mRNA 递送至抗原呈递细胞内,以完成人体对于免疫性抗原的高效识别。 LNP 体系还被广泛应用于癌症疫苗、寡核苷酸及基因编辑工具的体内递送。现有...
导读:2024年5月,西奈山伊坎医学院Nicolas Vabret团队在Cell子刊STAR Protocols分享实验方法:《Protocol for the development of mRNA lipid nanoparticle vaccines and analysis of immunization efficiency in mice》。文章详细描述了mRNA疫苗的制备和体内外活...
[1]. Kim B, Hosn RR, Remba T, Yun D, Li N, Abraham W, Melo MB, Cortes M, Li B, Zhang Y, Dong Y, Irvine DJ. Optimization of storage conditions for lipid nanoparticle-formulated self-replicating RNA vaccines. J Control Release. 2023 Jan;353:241-253. ...
Elucidation of lipid nanoparticle surface structure in mRNA vaccines ArticleOpen access05 October 2023 Introduction Lipid nanoparticles (LNPs) formulated from a mixture of an ionizable lipid, a helper lipid, cholesterol, a PEG lipid, and therapeutic nucleic acids have been shown to be potent and saf...
A drawback of the current mRNA-lipid nanoparticle (LNP) COVID-19 vaccines is that they have to be stored at (ultra)low temperatures. Understanding the root cause of the instability of these vaccines may help to rationally improve mRNA-LNP product stability and thereby ease the temperature condi...
脂质纳米颗粒(lipid nanoparticle, LNP)具有易于配制、稳定性高、细胞摄取高效、内体逃逸等优点, 是被公认的最成熟的递送载体。然而, LNP在肝脏中的积累严重限制了mRNA-LNP技术在肝脏以外的靶向与治疗。为了克服这一障碍, 研究人员一直专注于通过各种手段以实现肝外组织...
2022年11月30号,美国麻省理工学院科赫综合癌症研究所的Byungji Kim等人在Journal of Controlled Release[IF: 11.467] 发表文章:《Optimization of storage conditions for lipid nanoparticle-formulated self-replicating RNA vaccines》。研究者采用了自复制的HIV mRNA疫苗repRNA-LNP,分析了温度、缓冲液和冷冻保护剂(蔗糖...