These findings highlight the importance of lipid design and help enhance the shelf-life of mRNA/LNP systems. Piperidine-based ionizable lipids avoid the formation of mRNA鈥搇ipid adduct that is responsible for the loss-of-function of mRNA and contribute to the long-term storage stability of ...
COVID-19 Lipid nanoparticle (LNP) Lyophilization mRNA Shelf life Storage stability Structure Vaccine 1. Introduction Of the many COVID-19 vaccines under development, the two vaccines that have shown the most promising results in preventing COVID-19 infection represent a new class of vaccine products...
15.Hassett, K.J.; Higgins, J.; Woods, A.; Levy, B.; Xia, Y.; Hsiao, C.J.; Acosta, E.; Almarsson, Ö.; Moore, M.J.; Brito, L.A. Impact of lipid nanoparticle size on mRNA vaccine immunogenicity. J. Control. Release 2021, 335, 237–246. 16.Henderson, M.I.; Eygeris...
所以若对后续转染细胞或小鼠有需求的实验者,可以参考该文献对缓冲液体系进行优化。 [1]. Kim B, Hosn RR, Remba T, Yun D, Li N, Abraham W, Melo MB, Cortes M, Li B, Zhang Y, Dong Y, Irvine DJ. Optimization of storage conditions for lipid nanoparticle-formulated self-replicating RNA vaccine...
We then consider key points for the clinical translation of lipid nanoparticle–mRNA formulations, including good manufacturing practice, stability, storage and safety, and highlight preclinical and clinical studies of lipid nanoparticle–mRNA therapeutics for infectious diseases, cancer and genetic disorders...
2022年11月30号,美国麻省理工学院科赫综合癌症研究所的Byungji Kim等人在Journal of Controlled Release[IF: 11.467] 发表文章:《Optimization of storage conditions for lipid nanoparticle-formulated self-replicating RNA vaccines》。研究者采用了自复制的HIV mRNA疫苗repRNA-LNP,分析了温度、缓冲液和冷冻保护剂(蔗糖...
脂质纳米颗粒(Lipid Nanoparticle, LNP)作为mRNA疗法或疫苗的优选递送载体,被称为递送疫苗有效物质进入预定轨道的“运载火箭”,是目前最具潜力的递送载体之一。 LNP通常由四种脂质组成,包含胆固醇、二硬脂酰磷脂酰胆碱(DSPC)、可电离脂质1、2-⼆亚油酸-3-⼆甲氨基丙烷(MC3)和PEG化脂质。其中,可电离脂质MC3的...
高效低成本可电离脂质在 mRNA 脂质纳米颗粒疫苗中的应用研究 《Biomaterials》:Cost-Effective Yet High-Performance Ionizable Lipids for mRNA-Lipid Nanoparticle Vaccines 编辑推荐: 为解决现有 mRNA 疫苗中商业化可电离脂质(ILs)合成步骤多、质控复杂及成本高的问题,研究人员通过一锅法 Ugi 四组分反应(Ugi-4CR)...
Lipid nanoparticles (LNPs) for delivery of mRNA usually contain ionizable lipid/helper lipid/cholesterol/PEG-lipid in molar ratios of 50:10:38.5:1.5, respectively. These LNPs are rapidly cleared from the circulation following intravenous (i.v.) administration, limiting uptake into other tissues. ...
PackGene offers a one-stop platform for GMP mRNA and LNP by providing plasmid production, mRNA synthesis, Lipid Nanoparticle(LNP) encapsulation, formulation development, IND filing assistance, fill and finish, and complete documentation.