Mechanistically, this neuro-immune crosstalk is achieved through a serotonin-5-HT receptor 7 (HTR7) signaling axis that ultimately facilitates the pDC-mediated differentiation of IgAB cells from IgDprecursors in the gut. Single-cell RNA-seq data further reveal novel patterns of bidirectional ...
et al. CD8+ T cells orchestrate pDC–XCR1+ dendritic cell spatial and functional cooperativity to optimize priming. Immunity 46, 205–219 (2017). Article CAS PubMed PubMed Central Google Scholar Bonifaz, L. C. et al. In vivo targeting of antigens to maturing dendritic cells via the DEC...
2I). Idh is the first of four oxidative steps within the TCA cycle, and Dld codes for a mitochondrially-localized protein which serves as a NAD+ oxidoreductase in the pyruvate dehydrogenase multienzyme complex (Pdhc; Pdc; Pdh). This complex is an associated set of three enzymes that ...
BAC: WCA (Oxoid; CM0643; see manufacturer's instructions); Agar, 1.5 g; PdCl, 1 mg, dissolve first in HCl; phenosafranine, 2.5 mg; set pH to 7.0. After autoclaving (121 °C, 15 min), add kanamycin, 50 µg ml–1; cysteine (0.05% wt/vol), dithiothreitol (DTT...
Coverslips were functionalized as previously described25. In brief, coverslips (Thermo Scientific, 3421) were washed in nuclease-free water, followed by an immersion in 100% ethanol (Koptec). Subsequently, coverslips were air dried and cleaned using a plasma cleaner on the high setting (PDC-001...
block based on the H&E-stained Visium sections. Visualizing spatial NMF activity and inferred cell-type densities showcases the colocalization of highly correlated factor-cell pairs. NMF, non-negative matrix factorization; pDC/cDCs, plasmacytoid/classical dendritic cells; NK cells, natural killer ...
Using RNA-sequencing (RNA-seq) approaches, we examined the transcriptome profiles of in vitro cultured primary astrocytes and microglia to overview the function of CXCR3 in the brain. The normal upregulation of the genes associated with immune responses during T. gondii infection was impaired by ...
proliferating vascular endothelial cells; LEC: lymphatic endothelial cells; VECp: proliferating lymphatic endothelial cells; M: macrophages; DC: dendritic cells; pDC: plasmacytoid dendritic cells; Mp: proliferating macrophages; NK/T: mixed natural killer cells and T cells; NKp: proliferating natural kil...
cDNA synthesis from 500 ng total RNA was performed using a ReverTra Ace qPCR RT Kit (Toyobo, Osaka, Japan). Quantitative real-time PCR (qPCR) was performed in cDNA samples using a Power SYBR Green Master Mix (Applied Biosystems, Foster City, CA, USA) and the ABI Stepone Plus Real-Time...
block based on the H&E-stained Visium sections. Visualizing spatial NMF activity and inferred cell-type densities showcases the colocalization of highly correlated factor-cell pairs. NMF, non-negative matrix factorization; pDC/cDCs, plasmacytoid/classical dendritic cells; NK cells, natural killer ...