Mechanistically, this neuro-immune crosstalk is achieved through a serotonin-5-HT receptor 7 (HTR7) signaling axis that ultimately facilitates the pDC-mediated differentiation of IgAB cells from IgDprecursors in the gut. Single-cell RNA-seq data further reveal novel patterns of bidirectional ...
et al. CD8+ T cells orchestrate pDC–XCR1+ dendritic cell spatial and functional cooperativity to optimize priming. Immunity 46, 205–219 (2017). Article CAS PubMed PubMed Central Google Scholar Bonifaz, L. C. et al. In vivo targeting of antigens to maturing dendritic cells via the DEC...
Understanding the molecular determinants that drive the efficacy of these agents, may open-up patient selection strategies to improve clinical outcomes for ROR1-targeted ADC/PDC therapy. We utilized a small-domain protein-drug-conjugate platform to develop a PDC Candidate Drug (ADP-c389) which ...
Additional comparisons of the sc-RNA-seq data showed that some of the most highly serotonin-induced genes in LPDC in vitro (Fig. 3E) exhibited reduced expression in pDC in vivo in Tph2fl/fl; Hand2-Cre mice vs control animals; these genes included TGF-β activator Lrrc32, DC activation ...
BAC: WCA (Oxoid; CM0643; see manufacturer's instructions); Agar, 1.5 g; PdCl, 1 mg, dissolve first in HCl; phenosafranine, 2.5 mg; set pH to 7.0. After autoclaving (121 °C, 15 min), add kanamycin, 50 µg ml–1; cysteine (0.05% wt/vol), dithiothreitol (DTT...
2I). Idh is the first of four oxidative steps within the TCA cycle, and Dld codes for a mitochondrially-localized protein which serves as a NAD+ oxidoreductase in the pyruvate dehydrogenase multienzyme complex (Pdhc; Pdc; Pdh). This complex is an associated set of three enzymes that ...
We uncover axes of cell differentiation that are not apparent from single-cell RNA-sequencing (scRNA-seq) data, such as early dorsal–ventral separation of esophageal and tracheal progenitor populations in the gut tube. Our method provides an approach for studying cell fate decisions in complex ...
block based on the H&E-stained Visium sections. Visualizing spatial NMF activity and inferred cell-type densities showcases the colocalization of highly correlated factor-cell pairs. NMF, non-negative matrix factorization; pDC/cDCs, plasmacytoid/classical dendritic cells; NK cells, natural killer ...
BAC: WCA (Oxoid; CM0643; see manufacturer's instructions); Agar, 1.5 g; PdCl, 1 mg, dissolve first in HCl; phenosafranine, 2.5 mg; set pH to 7.0. After autoclaving (121 °C, 15 min), add kanamycin, 50 µg ml–1; cysteine (0.05% wt/vol), dithiothreitol (DTT) (0.02%), ...
Pdxdc1 protein is knocked down by shRNA in vivo The two anti-Pdxdc1 shRNAs (V2 and V3) were effective in knocking down Pdcxdc1. Infusion of V2, V3 or control shRNA into Figure 3. Pdxdc1 mRNA and protein expression in mouse brain. (a) Sample immunohistochemical (IHC) and in situ ...