Mechanistically, this neuro-immune crosstalk is achieved through a serotonin-5-HT receptor 7 (HTR7) signaling axis that ultimately facilitates the pDC-mediated differentiation of IgAB cells from IgDprecursors in the gut. Single-cell RNA-seq data further reveal novel patterns of bidirectional ...
FateID infers cell fate bias in multipotent progenitors from single-cell RNA-seq data. Nat. Methods 15, 379–386 (2018). Article CAS PubMed Google Scholar Inlay, M. A. et al. Ly6d marks the earliest stage of B-cell specification and identifies the branchpoint between B-cell and T-...
For mouse RNAseq N = 3 different animals, for human RNAseq N = 4 independent experiments, for mouse proteomics N = 4 different animals and for human proteomics N = 5 independent experiments. For RNAseq data p-values were determined by the Wald test and multi testing ...
BAC: WCA (Oxoid; CM0643; see manufacturer's instructions); Agar, 1.5 g; PdCl, 1 mg, dissolve first in HCl; phenosafranine, 2.5 mg; set pH to 7.0. After autoclaving (121 °C, 15 min), add kanamycin, 50 µg ml–1; cysteine (0.05% wt/vol), dithiothreitol (DTT...
We uncover axes of cell differentiation that are not apparent from single-cell RNA-sequencing (scRNA-seq) data, such as early dorsal–ventral separation of esophageal and tracheal progenitor populations in the gut tube. Our method provides an approach for studying cell fate decisions in complex ...
BAC: WCA (Oxoid; CM0643; see manufacturer's instructions); Agar, 1.5 g; PdCl, 1 mg, dissolve first in HCl; phenosafranine, 2.5 mg; set pH to 7.0. After autoclaving (121 °C, 15 min), add kanamycin, 50 µg ml–1; cysteine (0.05% wt/vol), dithiothreitol (DTT) (0.02%), ...
2.8. RNA-Seq Analysis Astrocytes and microglia were infected with T. gondii tachyzoites for 24 h at a multiplicity of infection (MOI) of 1.0. Infected and uninfected groups of cells were prepared in triplicate wells for each mouse genotype and each cell type. Total RNA was extracted with ...
RNA-seq analysis performed on the saliva of a breast cancer patient demonstrated a high expression of MMTV RNA in comparison to negative controls. It is noteworthy that: (a) the absence of viral sequences in the saliva of newborns eliminates the possibility of a trans-placental transmission and...
embryo implantation;mouse;single-cell RNA-seq;transcriptional changes 1. Introduction Embryo implantation is a crucial step for human reproduction. This is a low-efficiency process and the implantation failure rate per menstrual cycle is approximately 70% [1,2]. In assisted reproductive technologies, ...
cDNA synthesis from 500 ng total RNA was performed using a ReverTra Ace qPCR RT Kit (Toyobo, Osaka, Japan). Quantitative real-time PCR (qPCR) was performed in cDNA samples using a Power SYBR Green Master Mix (Applied Biosystems, Foster City, CA, USA) and the ABI Stepone Plus Real-Time...