1 beta, iNOS, TNF-alpha and IL-6 genes for M1 macrophages was significantly increased at 1.5 h after probiotic Ba treatment compared to the probiotic Ba-free treatment (P < 0.01), whereas the expression of M2 macrophage marker genes (Arg1, Fizz1, MR, Ym1) was decreased (P < 0.05). ...
Following M1 macrophage infiltration, macrophages switch to an anti-inflammatory M2 phenotype. In vitro, it has been shown that M1 to M2 phenotype switch is obtained after phagocytosis of either apoptotic or necrotic myogenic cells. Specifically, M1 macrophages show decreased expression of iNOS, CCL3...
小鼠肺中也有两类巨噬细胞,分为肺泡巨噬细胞(Alveolar macrophages)和肺间质巨噬细胞(Interstitial macrophages),可以用CD64+MerTK+圈出肺巨噬细胞,再通过CD11b和CD11c区分肺泡和肺间质巨噬细胞,且有利于在一个panel中同时做巨噬和...
Briefly, 4-μm-thick formalin-fixed, paraffin- embedded tissue sections were prepared and stained with hematoxylin and eosin or anti-CD68 (pan-macrophage marker), anti-CD80 (M1 macrophage marker), and anti-CD206 (M2 macrophage marker) antibodies, respectively (BioLegend, San Diego, CA, USA)...
Representative images showed immunofluorescent staining for the macrophage marker CD68 (green) and Arg1 (red) in the infarct myocardium. In the high- magnification view of the rectangle, Arg1-expressing CD68 ( þ ) macrophages (yellow) near DAPI-labeled MSCs (blue) were observed in the infarct...
Elevation of M2 macrophage marker in tumor-bearing mice. QPCR analysis of the mRNA expression of the markers of (A) M2 macrophages, mannose-receptor (mann-R) and (B) M1 macrophages, CCL3, in RNA extracted from peritoneal lavages or ascites fluid. Values were normalized to corresponding level...
Macrophage inflammatory protein 2 RANTES: Regulated upon activation normal T cell expressed and presumably secrete MTX: Methotrexate M1: M1 macrophages M2: M2 macrophages Th: T helper Treg: T regulatory cells SEM: Standard error of the mean ANOVA: One-way analysis of variance cAMP:...
小鼠肺中也有两类巨噬细胞,分为肺泡巨噬细胞(Alveolar macrophages)和肺间质巨噬细胞(Interstitial macrophages),可以用CD64+MerTK+圈出肺巨噬细胞,再通过CD11b和CD11c区分肺泡和肺间质巨噬细胞,且有利于在一个panel中同时做巨噬和DC的区分(不做详细划分巨噬细胞类型,也可用CD11b和F4/80marker检测)。后面还可以...
HMPL-013 alleviates CNV-induced macrophage M1 polarization HMPL-013 inhibited VEGF and VEGFR2 binding in macrophages infiltrating inside the CNV region. Then what was the effect of HMPL-013 on macrophage polarization? CNV-induced NOS2 (a M1-type macrophage marker) expression was inhibited (Fig. 4a...
M1 and M2 Macrophages: Oracles of Health and Disease. Crit Rev Immunol 32, 463–488 (2012). Article CAS PubMed Google Scholar Mantovani, A. et al. The chemokine system in diverse forms of macrophage activation and polarization. Trends Immunol 25, 677–686, doi: 10.1016/j.it.2004.09.015...