M1 macrophage-related gene model for NSCLC immunotherapy response predictiondoi:10.3724/abbs.2023262Sifan WuQiqi ShengPengjun LiuZhe JiaoJinru LvRong QiaoDongkun XieZanhan WangJiamei GePenghui LiActa Biochimica et Biophysica Sinica
Initially, the M1/M2 macrophage phenotype marker genes induced by IFNγand LPS (M1 inducer) were ana- lyzed using RT-PCR analysis. Treatment with IFNγ/LPS significantly up-regulated the mRNA levels of the M1 marker gene iNOS or IL12 in Ana-1 and RAW264.7 macrophages (P ≤...
widely involved in the pathophysiological processes in the human body, including fibrosis. However, its role in regulating macrophage-related inflammation in the fibrotic process of prostatitis has not been confirmed. Here, we aim to describe the role of CXCR4 in modulating macrophage...
Flow cytometry was used to detect M1 macrophage-specific surface marker CD80 expression levels. M1-induced macrophages were co-cultured with liver cancer MHCC97H cells using Transwell non-contact small sized co-culture dishes. MHCC97H cells invasion and metastasis were detected by Transwell and ...
1A). Initially, the M1/M2 macrophage phenotype marker genes induced by IFNγ and LPS (M1 inducer) were analyzed using RT-PCR analysis. Treatment with IFNγ/LPS significantly up-regulated the mRNA levels of the M1 marker gene iNOS or IL12 in Ana-1 and RAW264.7 macrophages (P ≤ ...
M1 macrophage marker expression. THP-1 macrophages were incubated with IFN-γ (20, 50, 100 ng) and/or LPS 10 pg/ml during 24 h. (a) mRNA expression of M1 macrophage markers was studied by RT-qPCR and normalized by RPS9 expression. Results are expressed as means ± 1 S.D. (n...
macrophage polarization detected by qRT-PCR 500 600 400 300 a 400 a 200 200 100 00 图注:转染 miR-378a 后 M1 型巨噬细胞相关因子 mRNA 表达明显降低, 与 M1 组比较,aP < 0.001 图 5 | qRT-PCR 检测各组 M1 型巨噬细胞标志蛋白的表达水平 Figure 5 | Expression level of marker genes in M1...
还有一个新鲜出炉(2023年8月)的science杂志文章:《CXCL9:SPP1 macrophage polarity identifies a network of cellular programs that control human cancers》,提出来的是CXCL9andSPP1(CS) expression but not by conventional M1 and M2 markers,而且是公布了数据:https://www.ncbi.nlm.nih.gov/geo/query/acc....
人组织相容性复合体Ⅰ类相关基因A(MICA)免疫试剂盒英文名称:Human major histocompatibility complex class I chain-related gene A,MICA ELISA Kit、组装 人组织多肽特异性抗原(TPS)免疫试剂盒英文名称:Human tissue polypeptide specific antigen,TPS ELISA Kit、分装 ...
RT-PCR analysis of mRNA expression of M1 and M2 MW-specific marker genes by test MWs was performed as follows. Total RNA was extracted from test MWs, including the MIS-MWs, BM-M1 MWs, and BM-M2 MWs, using the ISOGEN kit (Nippon Gene). After DNase-I treatment, the resultant RNA ...