2c,d). Also the recruitment of macrophages into SM tissue was confirmed by immunohistochemical staining for rodent macrophage marker (F4/80). As shown in Fig. 2e, albeit HFD-fed mice had strongly positive intense staining of F4/80, but HFD + RES mice had lower positive staining, ...
Flow cytometry was used to detect M1 macrophage-specific surface marker CD80 expression levels. M1-induced macrophages were co-cultured with liver cancer MHCC97H cells using Transwell non-contact small sized co-culture dishes. MHCC97H cells invasion and metastasis were detected by Transwell and ...
RT-PCR analysis of mRNA expression of M1 and M2 MW-specific marker genes by test MWs was performed as follows. Total RNA was extracted from test MWs, including the MIS-MWs, BM-M1 MWs, and BM-M2 MWs, using the ISOGEN kit (Nippon Gene). After DNase-I treatment, the resultant RNA ...
Compared with the control group, the expression of CX3CL1 and the M1 macrophage marker (iNOS) were significantly increased in the intestinal tissue of AS patients with IBD (Fig. 2C–E). The expression level of CX3CL1 was positively correlated with iNOS in the intestinal tissue of AS ...
In the coculture model, monocytes and M1 macrophages reduced transepithelial resistance as a marker for epithelial barrier integrity. The mechanisms for paracellular leakage included intracellular relocalization of tight junction proteins like claudin-2 and epithelial cell apoptosis. Determined by specific ...
M1型巨噬细胞的表面,表达TLR2、TLR4、CD80、CD86、INOS和MHC-II等Marker,同时M1型巨噬细胞可以释放TNF-α、IL-1β、IL-6、IL-12、CXCL9等细胞因子和趋化因子,通过调控NFkB、STAT1、STAT5、IRF3等转录因子信号调节实现。 相关细胞因子: 02 M2型巨噬细胞极化 ...
(B–I) RT-PCR analysis of M1 macrophage marker (iNOS, IL-6,IL-1β, TNF-α) and M2 macrophage marker (CD206, Arg1, IL-10 and iNOS) mRNA expression in each group (n = 4). (J–K) cardiac cell was stained with CD11b and MHC-II for flow cytometry analysis and quantification...
We also evaluated the AE9a Carm1Δ/Δ;Vav1-Cre+ cells for the expression of a macrophage-specific marker, F4/80, and found a significant increase in its expression in the Carm1Δ/Δ;Vav1-Cre+ mice compared with the Carm1Fl/Fl;Vav1-Cre− cells, after the second replating (Figure ...
Bone marrow-derived macrophages (BMDMs) were isolated and identified based on specific marker expression, and stimulated to differentiate into M1 macrophages with GM-CSF, IFN-gamma, and LPS. These cells were co-cultured with DPCs to evaluate the effect on macrophage differentiation. DPCs expressed ...
M1 is to macrophages as Th1 is to lymphocytes; among other cytokines, Th1 cells can produce high levels of IFN-γ, an extremely potent macrophage chemoattractant, and also one of the most potent endogenous M1 macrophage activation stimuli. LPS (endotoxin) from bacteria can also stimulate M1 (or...