M2 macrophage polarization is driven by transcription factors such as STAT6, peroxisome proliferator-activated receptor gamma (PPAR-γ), and Krüppel-like factor 4 (KLF4). IL-4 and IL-13 activate STAT6, which promotes the expression of M2-associated genes, such as arginase 1 (ARG1), mannose...
但大家可能还是有点迷糊,不知道实际应该如何应用,没事,以人巨噬细胞为例,可参考《Regulation of Human Macrophage M1–M2 Polarization Balance by Hypoxia and the Triggering Receptor Expressed on Myeloid Cells-1[3]》吧,里面有幅...
TREM2联合SPP1,去和FOLR2基因的排他性 还有一个新鲜出炉(2023年8月)的science杂志文章:《CXCL9:SPP1 macrophage polarity identifies a network of cellular programs that control human cancers》,提出来的是CXCL9andSPP1(CS) expression but not by conventional M1 and M2 markers,而且是公布了数据:https://...
M2 macrophage marker mRNA expression. (a) THP-1 macrophages were incubated during 24 h either with IL-4 and IL-13 (20 ng/ml each) or with IFN-γ 20 ng/ml and LPS 10 pg/ml and mRNA expression of M2 macrophage markers was studied by RT-qPCR and normalized by RPS9 expression. Result...
stimuli, in a manner so similar to the T lymphocytes of the adaptive immune branch that the “Th1 vs. Th2” nomenclature has been adopted to classify macrophage activation as “M1 vs. M2.” “M1” activation describes a macrophage performing “classical” anti-microbial functions, while “M2...
M1 and M2 macrophage polarization marker mRNA expression in trans-well co-cultured macrophages.doi:10.1371/JOURNAL.PONE.0085037.G004A. AnnaM. JenniferE. LindsayPLOS ONE
M1/M2 balance is skewed in sun-exposed aged skin To determine aging-related changes in dermal macrophage phenotypes in sun-exposed skin, we stained human skin tissue samples for CD86, a human M1 macrophage surface marker; CD206, a human M2 macrophage surface marker; and CD68 and CD11b, whic...
After selecting an appropriate dose, real-time fluorescence quantitative PCR (qRT-PCR) was used to analyze the expression of M1 macrophage marker genes [Interleukin (IL) -1β, IL-6, tumor necrosis factor- α (TNF- α), inducible nitric oxide synthase (iNOS)] and M2 macrophage marker genes...
We further confirmed these data by flow cytometry and show that M1 and M2 macrophages can be distinguished by their relative expression of CD38 and Egr2. Egr2 labeled more M2 macrophages (~70%) than the canonical M2 macrophage marker Arginase-1, which labels 24% of M2 macrophages. Conversely...
ADMSC~ExodecreasedM1~macrophagemarkerexpressionsofIL~6ꎬ TNF~αandCD86ꎬ andincreasedM2~macrophagemarkerexpressionsofArg~1ꎬ CD206andIL~10simultaneously. Conclusion ADMSC~ExomayinduceM1macrophagestodifferentiatetowardsM2macrophages.[Keywords] Mesenchymalstemcellsꎻ Exosomesꎻ Macrophagesꎻ Miceꎬ ...