Fig 3: Subset of M1 and M2 macrophages Antibodies to Analyze M1 & M2 Macrophage Subsets M1M2aM2bM2cM2d Stimulation/ActivationIFN-gammaIL-4IL-1BIL-10IL-6 LPSIL-13IL-1RTGF-betaAdenosine Marker ExpressionCD86CD163CD86CD163VEGF CD80MHC II ...
但大家可能还是有点迷糊,不知道实际应该如何应用,没事,以人巨噬细胞为例,可参考《Regulation of Human Macrophage M1–M2 Polarization Balance by Hypoxia and the Triggering Receptor Expressed on Myeloid Cells-1[3]》吧,里面有幅...
Methods:Immunohistological studies with Abs of pan-macrophage marker (CD68), M1 macrophage marker (CD80) and M2 macrophage marker (CD206) were performed on 24 surgical specimens obtained during a pars plana vitrectomy from 9 IVB-treated eyes, while 15 remained untreated. Cellular density of each...
The observations related to VD from this study, such as the decrease in M1 and M2 macrophage markers by calcitriol or their negative correlation with CYP27B1, suggest a modulatory, anticancer activity of VD in the tumor microenvironment (TME). However, the extent of these effects varied among ...
M1型巨噬细胞的表面,表达TLR2、TLR4、CD80、CD86、INOS和MHC-II等Marker,同时M1型巨噬细胞可以释放TNF-α、IL-1β、IL-6、IL-12、CXCL9等细胞因子和趋化因子,通过调控NFkB、STAT1、STAT5、IRF3等转录因子信号调节实现。 相关细胞因子: 02 M2型巨噬细胞极化 ...
electroacupuncture alleviates the inflammatory response via effects on m1 and m2 macrophages after spinal cord injury.[2.电针刺激减轻炎症反应通过影响脊髓损伤后m1和m2巨噬细胞。[ 低剂量照射诱导巨噬细胞向M2型极化 一种M1型巨噬细胞的鉴定方法与应用 烟曲霉孢子诱导M1型巨噬细胞极化的机制研究 四种不同来源巨...
ConclusionActivation of PPAR-γ receptor can promote the transformation of M1-type THP-1 macrophages to M2 type, reduce the secretion of inflammatory factors, and reduce the lipid deposition of macrophages. KEYWORDS: Peroxisome proliferator-activated receptor-γ;Macrophage polarization;Lipid metabolism Corr...
tumor cells of the bladder tumor treated by M2 macrophage showed an increase in the protein and mRNA expression of epithelial marker e-cadherin and zo-1, while the protein and mRNA expression of the interstitial marker vimentin and n-cadherind decreased. (3) the nf-kappa B pathway activa...
stimuli, in a manner so similar to the T lymphocytes of the adaptive immune branch that the “Th1 vs. Th2” nomenclature has been adopted to classify macrophage activation as “M1 vs. M2.” “M1” activation describes a macrophage performing “classical” anti-microbial functions, while “M2...
After selecting an appropriate dose, real-time fluorescence quantitative PCR (qRT-PCR) was used to analyze the expression of M1 macrophage marker genes [Interleukin (IL) -1β, IL-6, tumor necrosis factor- α (TNF- α), inducible nitric oxide synthase (iNOS)] and M2 macrophage marker genes...