Mouse Cre-LoxP system: general principles to determine tissue-specific roles of target genes. Lab Anim Res. 2018;34(4):147-159. DOI:10.5625/lar.2018.34.4.147 Missirlis PI, Smailus DE, Holt RA. A high-throughput screen identifying sequence and promiscuity characteristics of the loxP spacer ...
while immunohistochemical staining of orbital tissues exhibited macrophage infiltration and muscle fibrosis in the GD mice, contrasting with the control group.Conclusions: Our novel mouse model for GD, which showed the histological features of GO, was successfully established using the Cr...
mimicking human GD, and characterized the histological changes in Graves' orbitopathy (GO) progression after a single injection.Materials and Methods: A Cre-loxP system-based mouse model was constructed by inserting the CAG-loxP-STOP-loxP-hTSHR A-subunit cassette into the Rosa26 locus of the mou...
In CREST, the Cre-LoxP system restricts barcoding in specific cell populations of interest。并将其应用在体内和体外的大脑发育中,揭示了很多新的发现。 3.Cre-driven clonal lineage tracing based on CRISPR barcoding 首先,这个系统是如何工作的呢。作者提到:The system comprises three elements: (1) a ...
and metastatic biology can be investigated. The use of GEMMs facilitates investigation of specific mutations under spatiotemporal control using theCre-LoxPsystem, among other systems. The most frequently used mouse models take advantage of pancreas-specific cre-lines (eg,Pdx1-CreorPtf1a-Cre...
Recently, new models for investigation of mast cell functions based on the /loxP recombination system have been published and results in these new models challenged findings of previous studies in kit-mutant . Herein we describe procedures central to mast cell-specific gene inactivation and the ...
The cre-loxPsystem has arguably become the standard method for generating conditional null alleles33,34,35. Here, we report a high-throughput scheme for the extensive characterization of cre excision activity in driver lines that ends in the dissemination of valuable data to the research community ...
We discuss practical issues that arise when using the Cre- loxP system, such as the use of proper controls, Cre efficiency, reporter expression efficiency, and Cre leakiness. Finally, we introduce other methods for conditional gene expression, including Flp recombinase and the tetracycline-inducible...
1c). Such leaky recombination in dark after PA-Cre expression is not acceptable for any in vivo applications as Cre-loxP recombination is irreversible. To address this issue, we looked to improve the Magnets-based PA-Cre system by reducing the background dark activity. Fig. 1: Comparison of...
Prototype chromosomal vector utilizes the Cre/loxP system for site-specific insertion of circular donor vector into the cloning site on the chromosomal vector (Fig. 2). Donor vector carries a gene of interest, loxP cassette, and a part of a drug-resistant marker gene. Chromosomal vector carries...