2011年,Emiko Suzuki和Manabu Nakayama从Cre重组酶的同源物中筛选出VCre和SCre,并分别为他们构建了VloxP/Vlox2272和SloxP/Slox2272。VCre/VloxP系统和SCre/SloxP系统,与Cre/loxP系统具有较低的同源性,互不影响,可以结合使用。 4. ΦC31/pSIO系...
[5] Sandlesh, P., et al., Uncovering the fine print of the CreERT2-LoxP system while generating a conditional knockout mouse model of Ssrp1 gene. PLoS One, 2018. 13(6): p. e0199785. [6] Ji Q., et al., Hematopoietic PBX-in...
Cre和loxP是什么 1981年,Nat Sternberg等报道了一种克隆自大肠杆菌P1噬菌体的蛋白——由343个氨基酸编码,38kDa的Cre(Cyclization Recombination Enzyme)重组酶,它能特异识别loxP位点,并介导loxP位点间的序列删除或重组。loxP(locus of X-overP1)位点长为34bp,包括两个13bp的反向重复序列和一个8bp的间隔区域。其中,...
Mouse Cre-LoxP system: general principles to determine tissue-specific roles of target genes. Lab Anim Res. 2018; 34(4): 147–159. 5. McLellan MA, Rosenthal NA, Pinto AR. Cre-loxP-Mediated Recombination: General Principles and Experimental Considerations. Curr Protoc Mouse Biol. 2017;7(1)...
研究人员首先对BAC克隆进行改造,在小鼠Myh11基因的起始密码子后插入Cre-ERT2表达框,并去除BAC载体骨架上的loxP和loxP511位点。之后将改造后的BAC注射到C57BL/6小鼠受精卵的原核中,使其随机插入基因组。然后将受精卵植入代孕母亲体内,获得携带转基因的后代。Myh11-CreERT2-RAD小鼠的构建工作由赛业生物完成。为了确定...
human GD, and characterized the histological changes in Graves' orbitopathy (GO) progression after a single injection.Materials and Methods: A Cre-loxP system-based mouse model was constructed by inserting the CAG-loxP-STOP-loxP-hTSHR A-subunit cassette into the Rosa26 locus of the mouse genome...
肿瘤研究中,Cre-loxP系统被广泛用于在特定组织或细胞类型中精确地敲除或激活致癌基因,从而研究其在癌症发生、发展和治疗中的作用。以下是一些在肿瘤研究中重要的Cre小鼠品系(依名称字母排序)及其参考文献: Alb-Cre and LSL-Ctnnb1 用途:研究肝癌 应用:
Mouse Cre-LoxP system: general principles to determine tissue-specific roles of target genes. Lab Anim Res. 2018;34(4):147-159. DOI:10.5625/lar.2018.34.4.147 Missirlis PI, Smailus DE, Holt RA. A high-throughput screen identifying sequence and promiscuity characteristics of the loxP spacer ...
Cre和loxP是什么 1981年,Nat Sternberg等报道了一种克隆自大肠杆菌P1噬菌体的蛋白——由343个氨基酸编码,38kDa的Cre(Cyclization Recombination Enzyme)重组酶,它能特异识别loxP位点,并介导loxP位点间的序列删除或重组。loxP(locus of X-overP1)位点长为34bp,包括两个13bp的反向重复序列和一个8bp的间隔区域。其中,...
In principle, two transgenic mouse models (providing two different genetic components, respectively) will be required to achieve time specific knock-out, knock-in, and activation of target genes in mice. The first step is to establish Cre-expressing mouse strain. The cre recombinase driven by spe...