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(TEM) (Mizushima et al., 2010). Mitochondria within double-membranedautophagosomescan be seen at early stages of mitophagy, while at the later stages, residual fragmented mitochondria can be recognised within single-membraned autolysosomes that tend to be more electron-dense (Eskelinen et al., ...
Moreover, PINK1 expression displayed a significant positive association with HOMA‐β indices, and TEM studies further confirmed reduced distortions in mitochondrial morphology in the metformin group only. Our observations underscore that metformin upregulates mitophagy and subsequently ameliorates the altered ...
TEM显示Miz1过表达增强了线粒体清除率,而PRDX6过表达可以阻断这种作用(图4J)。 5. Miz1降解导致PRDX6解离并易位到Parkin Cys431,在NASH阶段抑制Parkin自身泛素化和Parkin介导的有丝分裂,而在NAFLD阶段则没有。 WDF诱导NASH模型的免疫沉淀显示,Parkin在8周时与PRDX6相互作用,然后增加,而MG132阻断了这种作用(图5A)...
通过透射电镜(TEM)检查了小鼠肝组织中的线粒体。Miz1缺失导致线粒体肿胀积聚,嵴结构异常(图3A)。从F/F和ΔHep小鼠中提取原代肝细胞(以下简称F/F和ΔHep原代肝细胞),并用游离脂肪酸(FFAs)刺激以模拟体内代谢应激的影响。免疫荧光显示Miz1可以影响线粒体积累,而耗氧比和细胞外酸化率分析显示Miz1不参与调节线粒...
a TEM of HeLa cells expressing gonococcal PorB from strain ATCC 49226 show mitochondrial capture in mitophagy-like structures. Data are mean ± s.d.; n = 21 cells, two-tailed Mann–Whitney test, P = 2 × 10−10. b Western blots showing degradation of mitochondrial ...
ROS: Reactive oxygen species TNF-α: Tumor Necrosis Factor-α TEM: Transmission Electron Microscope References Li RY, Guo L. Exercise in diabetic nephropathy: protective effects and molecular mechanism. Int J Mol Sci. 2024;25(7):3605. Article CAS PubMed PubMed Central Google Scholar Rai...
These changes are typically studied at the confocal and TEM microscopes and quantified through a morphometric approach. Due to their polarized nature, neurons present a loss in the neuritic domain more pronounced in respect to the cell body compartment. In addition, morphometric analysis show that ...
36). Furthermore, TEM images revealed that the SHC4H+hv group significantly promoted numerous double-membrane vesicles (autophagosomes) and concomitantly decreased unilamellar vesicles (autolysosomes) compared to the SHC4+hv group (Fig. 5c, d), resulting from the autophagic stress. Both ...
ATP5B and LC3B in mice aorta tissues. Mice were intratracheally instilled with 2.5 mg/kg or 5 mg/kg CuONPs for 3 days. Actin was used as the internal control.ERepresentative TEM images of mice abdominal aorta dissected from CuONPs-treated mice. Mice were treated with UA (30 mg/kg) fo...