The plasma in the supernatant was collected and stored at minus eighty degrees Celsius. Creatinine (Cre, Jiancheng Bioengineering Institute, C011-2-1) and blood urea nitrogen (BUN, Jiancheng Bioengineering Institute, C013-2-1) levels were measured using commercial reagent kits, and the data were...
For the first 10 days, cells were treated with SB431542 (10 mM; Tocris Bioscience) and Noggin (100 ng/ml) in Neural Media (NM) containing Neurobasal and DMEM (1:1), B27 supplement minus vitamin A (50 Â , Invitrogen), N2 supplement (100 Â , Invitrogen), GlutaMax (Invitrogen, ...
It is calculated as the value of the maximal respiration minus the basal respiration. ATP-linked respiration: Measurement of the cells' oxygen consumption coupled to ATP production. It is calculated as the value of the last rate measurement before oligomycin injection minus the minimum rate ...
For SILAC labeling, HeLa SCR and PUM2sh #1 clones were cultured in for six passages in SILAC DMEM media minus l-arginine and l-lysine (Thermo Scientific, 89985) with 10% dialyzed fetal bovine serum (Invitrogen) and 100 U/mL penicillin/streptomycin. Two biological replicates were performed, ...
The secondary antibody (anti-rabbit PLUS and anti-mouse MINUS) PLA probes were incubated at 1:5 dilution for 1 h at 37°C, followed by incubation in the ligation solution for 30 min at 37°C. Rolling circle amplification was done by incubating in the amplification-polymerase solution for...
The staining intensity was then calculated as the integrated density minus the background intensity. The values (expressed as Area %) from each measurement were averaged and expressed as the mean ± standard error [40]. 2.13. Enzyme-linked immunosorbent assay (ELISA) The activity levels of ...