本发明公开了一种用于检测miR8743p表达的引物,试剂盒,检测方法及应用,属于肿瘤生物治疗技术领域.本发明通过试验证实miR8743p与长春新碱的耐药性相关,在长春新碱耐药细胞中高表达,敏感细胞中低表达,因此可以作为肿瘤细胞长春新碱耐药的标志物.根据该标志物设计的引物,试剂盒及检测方法可用于临床判断肿瘤细胞对长春新碱...
miR‐874‐3pLong non‐coding RNAs (lncRNAs) have drawn growing attention because of the role which they play in various diseases, including colorectal cancer (CRC). However, the potential functions of lncRNA MCF2L antisense RNA 1 (MCF2L‐AS1) in tumors remained largely unclear. The present ...
miR‐874‐3P/ATG16L1 axisAutophagy is an important mechanism involved in the regulation of acute myeloid leukemia (AML) chemoresistance. The long noncoding RNA (lncRNA) DANCR exhibits oncogenic activity in several types of human cancers, including AML, but it remains unclear whether it regulates ...
Besides, siRNA knock down of XIAP in SKOV3-DDP cells had an anti-migratory effect like those with miR-874 overexpression. Importantly, the enforced expression of XIAP rescued SKOV3-DDP cells from the cytotoxic effects of miR-874-3p. Finally, miR-874-3p sensitized EOC cells to cisplatin-...
该研究进一步发现全身性敲除miR-501的小鼠,表现为树突结构缺陷、兴奋性谷氨酸传递增强,并出现社交缺陷、记忆障碍和感觉门控通道的异常表型;而当miR-501-3p的表达在神经元中恢复后,上述异常表型得到显著改善。通过对小鼠皮层蛋白质组学分析,发现...
图5C:qRT-PCR检测miR-199a-3p在MSCsSC-Exos处理的软骨细胞中的相对表达。 图5D:MSCsSC-Exos和antagomiR-199a-3p对IL -1β诱导的软骨细胞荧光素酶活性的影响。 图5E:mTOR mRNA 3′UTR中miR-199a-3p的预测结合位点示意图,以...
实时定量PCR检测施万细胞中过表达miR-148b-3p模拟物后靶基因的表达变化.结果 :miR-148b-3p能明显促进原代培养的施万细胞的增殖,miR-148b-3p过表达可抑制其靶基因Alcam,Cpd,Dedd,Nptx2,Phf20,Ptpn14和Stard 13的表达.结论 :miR-148b-3p有可能通过对增殖相关靶基因的负调控来影响施万细胞的增殖能力,从而促进...
miR-874-3p增殖凋亡背景长链非编码RNA(long non-coding RNA,lncRNA)在直肠癌(rectal cancer,RC)中异常表达并可能参与肿瘤发生及发展过程,lncRNA DCST1-AS1在肿瘤中表达上调,但其在RC发生及发展过程中的作用机制尚未阐明,假设lncRNA DCST1-AS1在RC细胞中表达水平升高,并可能促进肿瘤发生及发展.目的研究lncRNA DCS...
该hsamiR8743p在活动性肺结核患者的外周血单个核细胞和结核菌感染的人单核细胞系THP1中显着上调,并且上调的hsamiR8743p可能在单核巨噬细胞中作用于ATG16L1mRNA调控巨噬细胞自噬参与对Mtb的感染过程.上调的hsamiR874对APTB患者早期诊断具有一定的意义.因此,利用qRTPCR方法检测hsamiR874在APTB患者中的表达情况可以...
The miR-874-3p/MMP2/MMP3 axis might also provide direction for future cancer and inflammation investigations.doi:10.1016/j.ejphar.2021.173891Qingxin Song aFan Zhang bKun Wang aZhi Chen aQuan Li aZude Liu aHongxing Shen aEuropean Journal of Pharmacology...