产品活性:hsa-miR-877-5p antagomir 是一种经过特殊化学修饰的成熟miRNA的互补单链,全链进行甲氧基修饰,在5'端和3'端分别有2个和4个硫代骨架修饰,并在3'端连接有高亲和性胆固醇修饰。miRNA antagomir通过特异性结合成熟miRNA,阻止miRNA与其靶基因的互补配对,从而抑制miRNA的功能。相比于miRNA inhibitor,miRNA ant...
抑制miR-877-5p后,BCR-ABL mRNA表达显著上调,而过表达miR-877-5p后,BCR-ABL mRNA表达显著降低(图5D)。同样,western blot显示抑制miR-877-5p可增强BCR-ABL蛋白水平,而过表达miR-877-5p则表现出相反的作用(图5E)。CCK-8(图5F, G...
结果 观察组血清miR -877-5p 表达低于对照组，血清IL -1β、IL -6、TNF -α水平高于对照组（P 均<0.01）。随着病情严重程度增加，NARDS 患儿血清miR -877-5p 表达逐渐降低，血清IL -1β、IL -6、TNF -α水平逐渐升高（P 均<0.01）。Spearman 相关分析显示，NARDS 患儿血清miR -877-5p 表达与氧指数...
抑制miR-877-5p后,BCR-ABL mRNA表达显著上调,而过表达miR-877-5p后,BCR-ABL mRNA表达显著降低(图5D)。同样,western blot显示抑制miR-877-5p可增强BCR-ABL蛋白水平,而过表达miR-877-5p则表现出相反的作用(图5E)。CCK-8(图5F, G)和集落形成实验(图5H, I)显示,抑制miR-877-5p刺激CML细胞增殖,而miR-877...
miR7 mimics or agomir﹎iR7 were used as therapeutic agents in OA in vitro and in vivo models, respectively. Cell viability and apoptosis were evaluated using CCKand TUNEL staining, respectively. qRT㏄CR and western blotting were used to measure gene and protein expression, respectively. Results ...
Effects of crocin on clopidogrel-induced gastric epithelial cells injury by regulating miR-877-5p 引用 收藏 分享 摘要 目的探讨藏红花素对氯吡格雷诱导的胃黏膜上皮细胞损伤的作用及分子机制。方法将人胃黏膜上皮细胞GES-1分为对照组,模型组(0.5 mmol·L-1氯吡格雷)和藏红花素低、中、高浓度(0.1、......
我们通过分析预测miR-877-5p的可能靶基因,结果发现FOXM1的3′-UTR存在与miR-877-5p的结合位点.综上所述,我们提出假说:miR-877-5p直接调控FOXM1从而抑制PDAC的发生和进展.目的探讨miR-877-5p在胰腺导管腺癌发展中的作用及潜在的分子机制.方法(1)采用实时荧光定量PCR(Quantitative real-time polymerase chain ...
目的探讨新生儿急性呼吸窘迫综合征(NARDS)患儿血清微小RNA-877-5p(miR-877-5p)表达变化及其临床意义.方法选择NARDS患儿95例(观察组),病情严重程度:轻度38例,中度35例,重度22例,28 d内临床结局:死亡21例,存活74例,同期选择健康新生儿40例作为对照组.采集所有研究对象外周静脉血,离心留取血清,采用实时荧光定量聚合酶...
Sci. 2023, 24(23), 16758; https://doi.org/10.3390/ijms242316758 ActionDateNotesLink article pdf uploaded. 25 November 2023 11:25 CET Version of Record https://www.mdpi.com/1422-0067/24/23/16758/pdf-vor article supplementary file uploaded. 25 November 2023 11:25 CET - https://www....
总的来说,我们证明了miR-218-5p过度表达的外泌体加速了生长期的发生,而球状培养提供了细胞治疗的潜在途径。miR-218-5p通过下调WNT信号抑制剂SFRP2来调节毛囊发育,从而促进β-连环蛋白形成正反馈回路。与目前的头发再生实践相比,本研...