在喂食MCDD的小鼠肝脏线粒体中检测到GNMT-CII相互作用减少,在抗miR-873-5P存在下恢复(图5D)。这些结果表明,在NAFLD过程中miR-873-5p的诱导调节了线粒体GNMT水平及其与CII的相互作用,进一步表明GNMT在线粒体中的作用。 图5 (A)GnMT的WB...
miR‐873‐5p相对表达水平显著高于正常培养的足细胞(P<0.05).转染miR‐873‐5p inhibitor的高糖培养的足细胞增殖能力强于转染NC inhibitor的高糖培养的足细胞(P<0.05),而凋亡细胞比例却低于转染NC inhibitor的高糖培养的足细胞(P<0.05).HNRNPK是miR‐873‐5p潜在靶点,在高糖培养的足细胞中HNRNPK表达水平与miR‐873...
货号:chi-miR-873-5p 原价:￥4030 现价:￥3098.38节省:￥931.62 规格:1 kits 说明:chi-miR-873-5p 特异性茎环引物试剂盒 ￥615 产品参数 ---说明书下载--- microRNAs(miRNAs)是一类长度约19~24nt的非编码单链RNA,通过碱基互补配对的方式与靶基因的29’UTR区部分或完全互补,剪切靶基因的转录产物或者抑制转录...
MiR-873-5p could function as a tumour suppressor in CRC. It could inhibit the growth, proliferation, migration and invasion of CRC cells; influence the cell cycle and enhance apoptosis of CRC cells. Bioinformatics and luciferase reporter analyses demonstrated that Jumonji domain-containing protein 8...
(Supplementary Fig.8A-C). To explore the importance of miR-873-5p in circ6834-regulated tumor suppression in NSCLC, miR-873-5p mimics were cotransfected with circ6834 overexpression plasmid into NSCLC cells. The proliferation and metastasis abilities of NSCLC cells were enhanced by miR-873-5p ...
The cellular miR-873-5p expression was normalized to U6. The relative expression was calculated using the following equation: relative gene expression= 2−(ΔCtsample − ΔCtcontrol). The primers are listed in Supplementary Table 1. Western blot Cells were lysed on ice for 30 min ...
miR-155-5p的下调增加了耐药细胞对外部药物的敏感度。与亲代骨肉瘤细胞相比,阿霉素耐药细胞系中miR-155-5p、p-AKT和p-mTOR的表达明显增加,抑制miR-155-5p可增加抑癌基因PTEN的表达,降低骨肉瘤细胞的致瘤性,协同促进了阿霉素诱导的自噬和细胞凋亡情况[25...
目的:利用TCGA和GEO数据库分析miR-193b-3p和miR-196b-5p在急性髓系白血病(AML)非M3发病机制中的作用.方法1.首先利用在线数据库GEO下载GSE10358,GSE30285,GSE37642芯片数据,利用R包获得共同差异表达的基因(DEGs),并对DEGs进行GO功能富集分析,KEGG通路富集分析和PPI蛋白质互作分2.然后利用在线数据库TCGA获得169个AML...
miR‐873‐5pSPC18tongue squamous cell carcinomaObjective To explore the effect of miR3 on proliferation, apoptosis, migration and invasion of tongue squamous cell carcinoma (TSCC) by targeting SEC11A. Methods TSCC tissues were collected and performed by qRT㏄CR and Western blotting to determine the...
miR-873-5p表达显著下降(P<0.05).沉默MIR4435-2HG可明显降低SK-N-SH细胞存活率,迁移数,侵袭数,Ki-67,CDK2,MMP-2和MMP-9蛋白表达,显著提高细胞凋亡率,Cleaved caspase-3,Cleaved PARP蛋白水平(P<0.05).MIR4435-2HG靶向调控miR-873-5p表达.转染miR-873-5p可抑制SK-N-SH细胞增殖,迁移,侵袭,Ki-67,CDK2...