方法:分离收集BALB/c小鼠BMSCs并采用FCM法进行鉴定;采用脂质体法将miR-196b-5p-模拟物(miR-196b-5p-mimic)或miR-196b-5p-抑制子(miR-196b-5p-inhibitor)转入BMSCs,从培养液中收集BMSCs分泌的外泌体并予以鉴定(透射电子显微镜...
EVs rich in miR-196b-5p mediate the crosstalk between PTECs and fibroblast during the development of renal fibrosis, which might be associated with STAT3/SOCO2 signaling pathway.doi:10.1136/bmjdrc-2019-001101Renzhi HuXuan LiChuan PengRuifei Gao...
抑制miR-196b-5p的表达可以显著减弱成肌细胞增殖能力.进一步的功能研究发现,过表达miR-196b-5p能够显著增加成肌标志基因MyoD,MyoG和MyHC的表达水平(P<0.05);MyHC免疫荧光染色结果显示miR-196b-5p可以促进成肌细胞肌管形成,表明miR-196b-5p可以促进成肌细胞分化.生物信息学预测和双荧光素酶实验证明miR-196b-5p...
ROC analyses using the expression of miR-196b-5p and miR-107 in the discovery cohort were used to select thresholds at a 90% sensitivity to classify OSC. For miR-196b-5p, this threshold was 1172.258, yielding a 93% sensitivity with a 56% specificity (Figure 4). For miR-107, the thre...
本研究在体外水平探讨了miR-196b-5p在成脂分化、脂质合成和脂质分解中的功能及其作用机制。方法1.在骨髓基质细胞系ST2及间充质干细胞系C3H10T1/2中,运用实时定量PCR(qRT-PCR)技术检测miR-196b-5p在转染miR-196b-5p mimics后的变化。在两个细胞系中升高或降低miR-196b-5p水平,成脂诱导分化后用油红O染色、...
ROC analyses using the expression of miR-196b-5p and miR-107 in the discovery cohort were used to select thresholds at a 90% sensitivity to classify OSC. For miR-196b-5p, this threshold was 1172.258, yielding a 93% sensitivity with a 56% specificity (Figure 4). For miR-107, the thre...
EVs rich in miR-196b-5p mediate the crosstalk between PTECs and fibroblast during the development of renal fibrosis, which might be associated with STAT3/SOCO2 signaling pathway. 展开 关键词: tubulointerstitial changes fibroblast fibrosis DOI: 10.1136/bmjdrc-2019-001101 ...
与Control或mimics NC组相比,miR-196-5p mimics组的细胞增殖能力明显升高(t分别为7.97、7.85,均P<0.05,图1A);细胞侵袭数目明显增加(t分别为23.21、23.82,均P<0.05,图1B),N-cadherin蛋白表达量明显上升(t分别为17.27、17.59,均P<0.05...
In particular, the differential dysregulation of miR-196b-5p and miR-200b-3p characterizes the miRNA profile of adult medulloblastoma and suggests potential targets for novel diagnostic, prognostic, or therapeutic strategies. 展开 关键词: adult medulloblastoma childhood medulloblastoma microRNA microRNA ...