另外分别将miR-145-5p mimics(mimics组)、mimics control(NC组)转染SK-Hep1细胞。取对数生长期细胞SMMC-7721,以1×106/mL浓度接种于6孔板中,分别将miR-145-5p mimics(mimics组)、miR-145-5p inhibitor(inhibitor组)及其相应空白载体转染至SMMC-...
目的分析miR-145-5p在牙龈卟啉单胞菌(Pg)诱导的体外牙周炎模型中通过转化生长因子β1(TGF-β1)/Smads通路对巨噬细胞极化的影响。方法使用Pg-脂多糖(LPS)刺激小鼠巨噬细胞Raw264.7诱导构建体外牙周炎模型,分为NC组(空白对照)、Pg-LPS刺激组、Pg-LPS刺激+miR-145-5pmimics转染组(miR-145-5p过表达)。在光镜下...
To further determine the functional relationship between SPATS2 and MiR-145-5p in regulating HCC progression, rescue experiments were executed. Overexpression of MiR-145-5p significantly inhibited SPATS2 expression in SMMC-7721 or HepG2 cells. However, co-transfection MiR-145-5p with SPATS2 overe...
Following miRNA mimics transfection, cell viability, apoptosis and cells migration were determined by Cell Counting kit-8, Annexin V-FITC/propidium iodide staining and Transwell analyses. The target of miR-145-5p was analyzed by luciferase reporter assay and western blot analysis. It was observed ...
The results of the miR-145-5p expression efficiency test in LNCaP cells showed that miR-145-5p mimics could effectively upregulate miR-145-5p expression, while the miR-145-5p inhibitor could decrease the expression of miR-145-5p (Figure 2(a)). The expression level of miR-145-5p in PC3 ...
Second, the enhancing effects of the miR-145-5p mimics or Sp1 inhibitors in the pemetrexed sensitivity of NSCLC tumors have not been demonstrated in vivo. One could also notice that the increasing fold of miR-145-5p in the mimic transfected A400 cells reached a thousand times, but the ...
3.2. Role of miR-145a-5p in Mouse Myoblast Differentiation In order to discover the role of miR-145a-5p during myogenic differentiation, we firstly transfected miR-145a-5p inhibitors, mimics, or negative control (NC) into C2C12 cells during myoblasts differentiation (Figure 2(a)). As shown...
Furthermore, miR-145-5p mimics reduced candidate target gene specificity protein 1 ( Sp1 ) and nuclear factor κ B ( NF-κB ) ( p65 ) both in mRNA and protein levels. Knockdown of Sp1 phenocopied the effects of miR-145-5p overexpression on cell cycle regulators, EMT and the expression ...
miR-145a-5p promoted M2 while inhibited M1 microglia polarization leading to the reduced oxidative stress-induced neuron damage. (A) BV2 cells were transfected with miR-145a-5p mimics or Ctrl and stimulated with PBS, LPS + IFN-γ or IL-4 for 24 h, respectively. The mRNA level of ...
2.2 上调miR-140-5p抑制HDAC4的表达 在MG-63细胞中转染miR-140-5p mimics,并通过qRT-PCR确认转染成功。结果如图2A所示,相比转染NC mimic组,转染miR-140-5p组的MG-63细胞内的miR-140-5p表达明显升高。我们进一步考察了上调miR-140-5p对HDAC4表达的影响,结果发现,相比于NC mimic转染组,转染miR-140-5p mimics也...