3.1MTT法检测葛根素溶液及微乳对MDCK- MDR1的毒性 与对照组比较,葛根素溶液质量浓度在50～300 μg/mL给药组细胞存活率差异无显著性(P>0.05);不同稀释倍数的空白微乳与葛根素微乳对MDCK-MDR1细胞均有一定毒性,细胞毒性取决于微乳稀释倍数,且呈现出...
人源P-gp高表达MDR1-MDCKⅡ细胞模型由于培养周期短,代与代之间均一性良好,常作为体外筛选P-gp底物和抑制剂的细胞模型,并可用于研究P-gp介导DDI发生的可能性. 本研究应用逆转录病毒感染MDCKⅡ细胞的方法建立人源P-gp高表达MDR1-MDCKⅡ细胞模型.应用此细胞模型研究了来源于25种临床常用中药的50种活性成分对P-gp...
描述: Inhibition of human MDR1-dependent accumulation of calcein-AM expressed in MDCK2 cells at 20 uM Format: BAO_0000219 Organism: Homo sapiens Cell type: MDCK-II Target: P-glycoprotein 1 (ALA4302) Document: ALA1153836 Inhibition of human MDR1-dependent accumulation of calcein-AM expressed...
Huang, Comparison of blood-brain barrier permeability assays: in situ brain perfusion, MDR1-MDCKII and PAMPA-BBB, J. Pharm. Sci. 98 (2009) 1980-1991.Di L, Kerns EH, Bezar IF, Petusky SL, Huang Y. (2009) Comparison of blood-brain barrier permeability assays: in situ brain perfusion,...
概述 了国内外关于利用MDCK—MDR!细胞系作为模型进行药物筛选、药物相互作用和研究药物吸收转运机制等方面的 型快速筛选P—gP底物和抑制剂,以及肠道、血脑屏障、。肾脏的体外模型。 关键词:MDCK—MDRl细胞;P一糖蛋白;药物转运;细胞模型 中图分类号:R965.1 文献标识码:A 文章编号:0513—4870(2008)06—0559—06 ...
通过建立并验证MDCKII-MDR1单层细胞体外药物转运模型,并以此细胞模型为工具,考察蛋白激酶C激动剂Phorbol-12-myristate-13-acetate(PMA)对P-gp介导的地高辛外排转运的... 黎玉华,毕惠嫦,钟国平,... - 全国临床药理学学术大会 被引量: 0发表: 2012年 蛋白激酶C激动剂对地高辛在MDCKII-MDR1细胞模型中转运的影响...
Verapamil displayed a vectorial basolateral-to-apical transepithelial efflux across the MDCK II MDR1 monolayers with a permeability of 5.7×105cmsec1 compared to an apical to basolateral permeability of 1.3×105cmsec-1. The efflux could be inhibited with the P-gp inhibitor zosuquidar. Zosuquid...
通过建立并验证MDCKII-MDR1单层细胞体外药物转运模型,并以此细胞模型为工具,考察蛋白激酶C激动剂Phorbol-12-myristate-13-acetate(PMA)对P-gp介导的地高辛外排转运的... 黎玉华,毕惠嫦,钟国平,... - 全国临床药理学学术大会 被引量: 0发表: 2012年 蛋白激酶C激动剂对地高辛在MDCKII-MDR1细胞模型中转运的影响...
For large-scale Pgp screening of new chemical entities (NCEs), often BBB surrogate models such as MDR1-transfected MDCKII (Madin-Darby canine kidney strain II) and LLC-PK1 (Lilly Laboratories cells—porcine kidney 1) cells or a human colon carcinoma cell line (Caco-2) are used as general...
(NCEs), often BBB surrogate models such asMDR1-transfected MDCKII (Madin-Darby canine kidney strain II) and LLC-PK1 (Lilly Laboratories cells—porcine kidney 1) cells or a human colon carcinoma cell line (Caco-2) are used as general barrier models, because these polarized epithelial cells ...