This study investigates an in vitro model using P-gp expressing MDCKII-MDR1 cells for predicting in vivo brain drug penetration. Methods: In vitro equilibrium distribution studies were conducted in apical and basolateral solutions with high protein content to estimate Kp,brain and Kp,uu,brain ...
人源P-gp高表达MDR1-MDCKⅡ细胞模型由于培养周期短,代与代之间均一性良好,常作为体外筛选P-gp底物和抑制剂的细胞模型,并可用于研究P-gp介导DDI发生的可能性. 本研究应用逆转录病毒感染MDCKⅡ细胞的方法建立人源P-gp高表达MDR1-MDCKⅡ细胞模型.应用此细胞模型研究了来源于25种临床常用中药的50种活性成分对P-gp...
Verapamil displayed a vectorial basolateral-to-apical transepithelial efflux across the MDCK II MDR1 monolayers with a permeability of 5.7×105cmsec1 compared to an apical to basolateral permeability of 1.3×105cmsec-1. The efflux could be inhibited with the P-gp inhibitor zosuquidar. Zosuquid...
Pharmacy sciences Effect of pharmaceuticals and natural products on multidrug resistance mediated transport in Caco-2 and MDCKII-MDR1 drug transport models OREGON STATE UNIVERSITY FanYingThis thesis details my investigation of some pharmaceuticals and naturalproducts on the transport of drugs which are ...
Polysorbates with one fatty acid chain decreased the efflux of digoxin through P-gp inhibition in MDCKII MDR1 cells. Mono- and di-saccharide-based surfactants, in a dose dependent manner, enhanced digoxin absorptive permeability without decreasing the secretory permeability in Caco-2 cells, ...
Three objectives were performed to address this goal.;The second objective was to investigate if specific flavonoids such as EGCG, epicatechin gallate (ECG), and xanthohumol can modulate the cellular uptake and the permeability of CSA and digoxin across the Caco-2 and MDCKII-MDR1 cell transport ...
Permeability data from MDR1-MDCKII and PAMPA-BBB assays were compared to data from in situ brain perfusion to evaluate the accuracy of in vitro assays in predicting in vivo blood–brain barrier (BBB) permeability. PAMPA-BBB significantly correlated to in situ brain perfusion, however, MDR1-MDCK...
Evaluation of Drug Transport in MDCKII-Wild Type, MDCKII-MDR1, MDCKII-BCRP and Caco-2 Cell LinesIntroductionPathophysiology of Hepatobiliary SystemInborn Errors of Bile Acid/Salt Transporter DeficienciesDrugs and Cholestatic Liver DiseasePhospholipases and PhospholipidosisMechanisms of Toxicity Associated ...
Comparison of blood- brain barrier permeability assays: in situ brain perfusion, MDR1-MDCKII and PAMPA-BBB. J. Pharm. Sci. 98, 1980-1991.L. Di, E.H. Kerns, I.F. Bezar, S.L. Petusky, Y. Huang, Comparison of blood-brain barrier permeability assays: in situ brain perfusion, MDR1...
Williams GC, Knipp GT, and Sinko PJ (2003) The effect of cell culture conditions on saquinavir transport through and interactions with, MDCKII cells overexpressing hMDR1. J Pharm Sci 92:1957-1967.The effect of cell culture conditions on saquinavir transport through, and interactions with, MDCK...